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Literature summary extracted from

  • Dons, R.F.; Doughty, C.C.
    Isolation and characterization of aldose reductase from calf brain (1976), Biochim. Biophys. Acta, 452, 1-12.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.21 SO42- stimulation Bos taurus
1.1.1.21 SO42- ammonium and lithium salt is somewhat more effective than sodium and potassium salt Bos taurus

General Stability

EC Number General Stability Organism
1.1.1.21 -55°C, unstable to prolonged storage Bos taurus
1.1.1.21 freezing and thawing: more than twice, unstable Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.21 29000
-
 isozyme AR I, gel filtration Bos taurus
1.1.1.21 30000
-
 isozyme AR II, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.21 aldose + NAD(P)H Bos taurus
-
 alditol + NAD(P)+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.21 Bos taurus
-
 calf
-
1.1.1.21 Bos taurus
-
 2 enzymes AR I (ALR 1) and AR II (ALR 2)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.21 2 enzymes: AR I and AR II Bos taurus
1.1.1.21 calf Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.21 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.21 0.488
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.21 alditol + NAD(P)+
-
 Bos taurus aldose + NAD(P)H
-
 r
1.1.1.21 aldose + NAD(P)H
-
 Bos taurus alditol + NAD(P)+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.21 monomer 1 * 30000, isozyme AR I, SDS-PAGE, monomer is in equilibrium with a less effective dimer Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.21 6.6
-
 DL-glyceraldehyde + NADPH Bos taurus
1.1.1.21 10
-
 glycerol + NADP+ Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.21 4.8 8.6 2 h, stable Bos taurus