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Literature summary extracted from

  • Nakagawa, M.; Tsukuda, F.; Nakayama, T.; Matsuura, K.; Hara, A.; Sawada, H.
    Identification of two dihydrodiol dehydrogenases associated with 3(17)alpha-hydoxysteroid dehydrogenase activity in mouse kidney (1989), J. Biochem., 106, 633-638.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.209 17beta-estradiol 5 mM, 77% inhibition Mus musculus
1.1.1.209 5alpha-Androstan-3beta-ol-17-one 1 mM, 80% inhibition Mus musculus
1.1.1.209 5alpha-pregnan-3beta-ol-21-one 1 mM, 66% inhibition Mus musculus
1.1.1.209 5beta-androstan-3beta-ol-17-one 1 mM, 91% inhibition Mus musculus
1.1.1.209 5beta-Pregnan-3beta-ol-20-one 1 mM, 80% inhibition Mus musculus
1.1.1.209 Barbital 100 mM, 11% inhibition Mus musculus
1.1.1.209 Dienstrol 1 mM, 22% inhibition Mus musculus
1.1.1.209 Hexestrol 1 mM, 77% inhibition Mus musculus
1.1.1.209 Stilbestrol 10 mM, 75% inhibition Mus musculus
1.1.1.209 testosterone 1 mM, 48% inhibition Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.209 0.0001
-
 Epitestosterone cofactor NADP+ Mus musculus
1.1.1.209 0.0001
-
 5beta-androstane-3alpha,17beta-diol cofactor NADP+ Mus musculus
1.1.1.209 0.0003
-
 5beta-androstane-3alpha,17beta-diol cofactor NADP+ Mus musculus
1.1.1.209 0.0004
-
 glycolithocholic acid cofactor NADP+ Mus musculus
1.1.1.209 0.0005
-
 5beta-pregnane-3alpha,20alpha-diol cofactor NADP+ Mus musculus
1.1.1.209 0.0007
-
 5beta-pregnane-3alpha-ol-20-one cofactor NADP+ Mus musculus
1.1.1.209 0.004
-
 NADP+
-
 Mus musculus
1.1.1.209 0.16
-
 (R)-1-indanol cofactor NADP+ Mus musculus
1.1.1.209 0.26
-
 NAD+
-
 Mus musculus
1.1.1.209 0.77
-
 trans-benzene dihydrodiol cofactor NADP+ Mus musculus
1.1.1.209 1.99
-
 cis-benzene dihydrodiol cofactor NADP+ Mus musculus
1.1.1.209 2.23
-
 cyclohex-2-ene-1-ol cofactor NADP+ Mus musculus
1.1.1.209 2.67
-
 Naphthalene dihydrodiol cofactor NADP+ Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.209 34000
-
 gel filtration Mus musculus
1.1.1.209 39000
-
 1 * 39000, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.209 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.209 Q-Sepharose, Matrex Red A, HA-Ultrogel, Sephadex G-100, two enzyme preparations DD1 and DD2 with almost identical properties, products of post-translational or post-mortem modification rather than isoenzymes Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.209 kidney
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.209 0.27
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.209 (R)-1-indanol + NADP+ 37% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 1-indanone + NADPH
-
 ?
1.1.1.209 (S)-1-indanole + NADP+ 7% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 1-indanone + NADPH
-
 ?
1.1.1.209 1-acenaphthenol + NADP+ 10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 1-acenaphthenone + NADPH
-
 ?
1.1.1.209 4-nitrobenzaldehyde + NADPH 58% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.209 5alpha-androstane-3alpha,17beta-diol + NADP+ 7% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 5alpha-androstane-17beta-ol-3-one + NADPH
-
 ?
1.1.1.209 5alpha-pregnan-3alpha,20alpha-diol + NADP+ 6% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 5alpha-pregnan-20alpha-ol-3-one + NADPH
-
 ?
1.1.1.209 5alpha-pregnan-3alpha,21-diol-20-one + NADP+ 7% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 5alpha-pregnan-21alpha-ol-3,20-dione + NADPH
-
 ?
1.1.1.209 5alpha-pregnane-21-ol-3,20-dione + NADPH 75% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 5beta-pregnane-3alpha,21-diol-20-one + NADP+
-
 ?
1.1.1.209 5alpha-pregnane-3,20-dione + NADPH 10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 5alpha-pregnane-3alpha-ol-20-one + NADP+
-
 ?
1.1.1.209 5beta-androstan-17beta-ol-3-one + NADPH 10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 5beta-androstan-17beta,3alpha-diol + NADP+
-
 ?
1.1.1.209 5beta-androstane-3,17-dione + NADPH 10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 5beta-androstan-3alpha-ol-17-one + NADP+
-
 ?
1.1.1.209 5beta-androstane-3alpha,17beta-diol + NADP+ 11% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 5beta-androstane-17beta-ol-3-one + NADPH
-
 ?
1.1.1.209 5beta-androstane-3alpha-ol-17-one + NADP+ 9% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 5beta-androstane-3,17-dione + NADPH
-
 ?
1.1.1.209 5beta-pregnan-3alpha,20alpha-diol + NADP+ 26% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 5beta-pregnan-3-one-20alpha-ol + NADPH
-
 ?
1.1.1.209 5beta-pregnan-3alpha-ol-20-one + NADP+ 19% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 5beta-pregnan-3,20-dione + NADPH
-
 ?
1.1.1.209 5beta-pregnane-3,20-dione + NADPH + H+ 21% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 5beta-pregnane-3alpha-ol-20-one + NADP+
-
 ?
1.1.1.209 acenaphthenequinone + NADPH 44% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus acenaphthenehydroquinone + NADP+
-
 ?
1.1.1.209 androst-4-ene-3,17-dione + NADPH 10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus androst-4-ene-3alpha-ol-17-one + NADP+
-
 ?
1.1.1.209 camphorquinone + NADPH + H+ 52% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus camphorhydroquinone + NADP+
-
 ?
1.1.1.209 cis-1,2-dihydroxy-3,5-cyclohexadiene + NADP+ 60% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus catechol + NADPH
-
 ?
1.1.1.209 cyclohex-2-ene-1-ol + NADP+ 9% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus cyclohex-2-en-1-one + NADPH
-
 ?
1.1.1.209 cyclohexanone + NADPH 16% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus cyclohexanol + NADP+
-
 ?
1.1.1.209 epitestosterone + NADP+ 11% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus testosterone + NADPH + H+
-
 ?
1.1.1.209 glycolithocholic acid + NADP+ 56% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus N-[(5beta)-3,24-dioxocholan-24-yl]glycine + NADPH
-
 ?
1.1.1.209 naphthalene dihydrodiol + NADP+ 49% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus naphthacatechol + NADPH
-
 ?
1.1.1.209 pyridine-4-ol + NADP+ 75% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene Mus musculus 3H-pyridine-4-one + NADPH
-
 ?
1.1.1.209 tetrahydrodeoxycorticosterone + NAD+
-
 Mus musculus ?
-
 r
1.1.1.209 trans-1,2-dihydroxy-3,5-cyclohexadiene + NADP+
-
 Mus musculus catechol + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.209 monomer 1 * 39000, SDS-PAGE Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.209 6 6.8 5beta-pregnane-3,20-dione as substrate, cofactor NADPH Mus musculus