BRENDA - Enzyme Database show

Comparison of the multiple forms of the soluble 3(17)alpha-hydroxysteroid dehydrogenases of female rabbit kidney and liver

Lau, P.C.K.; Layne, D.S.; Williamson, D.G.; J. Biol. Chem. 257, 9450-9456 (1982)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.209
androsterone
competitive vs. epitestosterone
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.209
0.00053
-
Epitestosterone
liver isozyme IB
Oryctolagus cuniculus
1.1.1.209
0.00061
-
Epitestosterone
kidney isozyme II
Oryctolagus cuniculus
1.1.1.209
0.0012
-
androsterone
liver isozyme IC
Oryctolagus cuniculus
1.1.1.209
0.0021
0.0024
Epitestosterone
liver isozyme IB, androsterone, kidney, isozyme IB
Oryctolagus cuniculus
1.1.1.209
0.00291
-
Epitestosterone
kidney isozyme IB
Oryctolagus cuniculus
1.1.1.209
0.004
0.0047
androsterone
kidney isozyme II, liver isozyme IB
Oryctolagus cuniculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.209
cytosol
-
Oryctolagus cuniculus
5829
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.209
Oryctolagus cuniculus
-
emale
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.209
kidney
-
Oryctolagus cuniculus
-
1.1.1.209
liver
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.209
17alpha-estradiol + NAD(P)+
-
286233
Oryctolagus cuniculus
estrone + NAD(P)H
-
286233
Oryctolagus cuniculus
r
1.1.1.209
17alpha-estradiol 3-glucuronide + NAD(P)+
-
286233
Oryctolagus cuniculus
estrone 3-glucuronide + NAD(P)H
-
286233
Oryctolagus cuniculus
r
1.1.1.209
3alpha-hydroxy-5alpha-androstan-17-one + NAD(P)+
-
286233
Oryctolagus cuniculus
5alpha-androstan-3,17-dione + NAD(P)H
-
286233
Oryctolagus cuniculus
?
1.1.1.209
5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
286233
Oryctolagus cuniculus
17beta-hydroxy-5alpha-androstan-3-one + NAD(P)H
i.e. 5alpha-dihydrotestosterone
286233
Oryctolagus cuniculus
r
1.1.1.209
epitestosterone + NAD(P)+
-
286233
Oryctolagus cuniculus
androst-4-en-3,17-dione + NAD(P)H + H+
-
286233
Oryctolagus cuniculus
r
1.1.1.209
estrone 3-sulfate + NAD(P)H
-
286233
Oryctolagus cuniculus
17alpha-estradiol 3-sulfate + NAD(P)+
-
286233
Oryctolagus cuniculus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.209
6
6.5
-
Oryctolagus cuniculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.209
NAD+
-
Oryctolagus cuniculus
1.1.1.209
NADH
low activity
Oryctolagus cuniculus
1.1.1.209
NADP+
preferred
Oryctolagus cuniculus
1.1.1.209
NADPH
preferred
Oryctolagus cuniculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.209
NAD+
-
Oryctolagus cuniculus
1.1.1.209
NADH
low activity
Oryctolagus cuniculus
1.1.1.209
NADP+
preferred
Oryctolagus cuniculus
1.1.1.209
NADPH
preferred
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.209
androsterone
competitive vs. epitestosterone
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.209
0.00053
-
Epitestosterone
liver isozyme IB
Oryctolagus cuniculus
1.1.1.209
0.00061
-
Epitestosterone
kidney isozyme II
Oryctolagus cuniculus
1.1.1.209
0.0012
-
androsterone
liver isozyme IC
Oryctolagus cuniculus
1.1.1.209
0.0021
0.0024
Epitestosterone
liver isozyme IB, androsterone, kidney, isozyme IB
Oryctolagus cuniculus
1.1.1.209
0.00291
-
Epitestosterone
kidney isozyme IB
Oryctolagus cuniculus
1.1.1.209
0.004
0.0047
androsterone
kidney isozyme II, liver isozyme IB
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.209
cytosol
-
Oryctolagus cuniculus
5829
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.209
kidney
-
Oryctolagus cuniculus
-
1.1.1.209
liver
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.209
17alpha-estradiol + NAD(P)+
-
286233
Oryctolagus cuniculus
estrone + NAD(P)H
-
286233
Oryctolagus cuniculus
r
1.1.1.209
17alpha-estradiol 3-glucuronide + NAD(P)+
-
286233
Oryctolagus cuniculus
estrone 3-glucuronide + NAD(P)H
-
286233
Oryctolagus cuniculus
r
1.1.1.209
3alpha-hydroxy-5alpha-androstan-17-one + NAD(P)+
-
286233
Oryctolagus cuniculus
5alpha-androstan-3,17-dione + NAD(P)H
-
286233
Oryctolagus cuniculus
?
1.1.1.209
5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
286233
Oryctolagus cuniculus
17beta-hydroxy-5alpha-androstan-3-one + NAD(P)H
i.e. 5alpha-dihydrotestosterone
286233
Oryctolagus cuniculus
r
1.1.1.209
epitestosterone + NAD(P)+
-
286233
Oryctolagus cuniculus
androst-4-en-3,17-dione + NAD(P)H + H+
-
286233
Oryctolagus cuniculus
r
1.1.1.209
estrone 3-sulfate + NAD(P)H
-
286233
Oryctolagus cuniculus
17alpha-estradiol 3-sulfate + NAD(P)+
-
286233
Oryctolagus cuniculus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.209
6
6.5
-
Oryctolagus cuniculus