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Literature summary extracted from

  • Hirano, S.; Masuda, N.
    Characterization of NADP-dependent 7beta-hydroxysteroid dehydrogenases from Peptostreptococcus productus and Eubacterium aerofaciens (1982), Appl. Environ. Microbiol., 43, 1057-1063.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.201 iodoacetate complete inhibition at 0.05 mM Blautia producta
1.1.1.201 p-chloromercuribenzoic acid complete inhibition at 0.001 mM Blautia producta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.201 0.022
-
 3alpha,7beta-dihydroxy-5beta-cholanoic acid
-
 Blautia producta
1.1.1.201 0.1
-
 NADP+
-
 Blautia producta
1.1.1.201 0.108
-
 3alpha,7beta-dihydroxy-5beta-cholanoic acid
-
 Collinsella aerofaciens
1.1.1.201 0.238
-
 3alpha,7beta-dihydroxy-5beta-cholanoyl glycine
-
 Blautia producta
1.1.1.201 0.286
-
 3alpha,7beta-dihydroxy-5beta-cholanoyl taurine
-
 Blautia producta
1.1.1.201 0.4
-
 NADP+
-
 Collinsella aerofaciens
1.1.1.201 0.909
-
 3alpha,7beta-dihydroxy-5beta-cholanoyl glycine
-
 Collinsella aerofaciens
1.1.1.201 1.639
-
 3alpha,7beta-dihydroxy-5beta-cholanoyl taurine
-
 Collinsella aerofaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.201 45000
-
 gel filtration Collinsella aerofaciens
1.1.1.201 53000
-
 gel filtration Blautia producta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ Collinsella aerofaciens ursodeoxycholic acid 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ Blautia producta ursodeoxycholic acid 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ Collinsella aerofaciens only active on free and conjugated bile salts 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ Blautia producta only active on free and conjugated bile salts 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+ Collinsella aerofaciens
-
 3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+ Blautia producta
-
 3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+ Collinsella aerofaciens
-
 3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+ Blautia producta
-
 3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.201 Blautia producta
-
-
-
1.1.1.201 Collinsella aerofaciens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.201 to homogeneity, chromatography techniques Collinsella aerofaciens
1.1.1.201 to homogeneity, chromatography techniques Blautia producta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.201 cell culture
-
 Collinsella aerofaciens
-
1.1.1.201 cell culture
-
 Blautia producta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.201 0.1
-
 crude cell extract Collinsella aerofaciens
1.1.1.201 0.17
-
-
 Collinsella aerofaciens
1.1.1.201 1.06
-
-
 Blautia producta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ ursodeoxycholic acid Collinsella aerofaciens 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ ursodeoxycholic acid Blautia producta 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ only active on free and conjugated bile salts Collinsella aerofaciens 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+ only active on free and conjugated bile salts Blautia producta 3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
-
 Collinsella aerofaciens 3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
-
 Blautia producta 3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
-
 Collinsella aerofaciens 3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
 ?
1.1.1.201 3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
-
 Blautia producta 3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.201 40
-
 50% loss of activity within 30 min Blautia producta
1.1.1.201 50
-
 90% loss of activity within 3 min Blautia producta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.201 9.1 11
-
 Collinsella aerofaciens
1.1.1.201 9.5 10.5
-
 Blautia producta

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.201 NADP+ no activity with NAD+ Collinsella aerofaciens
1.1.1.201 NADP+ no activity with NAD+ Blautia producta