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Literature summary extracted from

  • Hayashi, S.; Watanabe, M.; Kimura, A.
    Enzymatic determination of free glucoronic acid with glucuronolactone reductase. I. isolation and purification of glucoronolactone reductase from rat kidney (1984), J. Biochem., 95, 223-232.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.20 freezing and thawing: 3 times causes 25% decrease of activity, 5 times causes 40% decrease of activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.20 Barbital
-
 Rattus norvegicus
1.1.1.20 barbiturates
-
 Rattus norvegicus
1.1.1.20 dithiothreitol
-
 Rattus norvegicus
1.1.1.20 Hg2+
-
 Rattus norvegicus
1.1.1.20 iodoacetic acid
-
 Rattus norvegicus
1.1.1.20 NaF
-
 Rattus norvegicus
1.1.1.20 Phenobarbital
-
 Rattus norvegicus
1.1.1.20 Zn2+
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.20 0.045
-
 NADPH with D-glucuronic acid Rattus norvegicus
1.1.1.20 4
-
 D-galacturonic acid
-
 Rattus norvegicus
1.1.1.20 6
-
 D-glucuronic acid L-iduronic acid Rattus norvegicus
1.1.1.20 9
-
 D-glucuronolactone
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.20 cytoplasm
-
 Rattus norvegicus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.20 40000
-
 1 * 40000, SDS-PAGE Rattus norvegicus
1.1.1.20 41000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.20 L-gulono-1,4-lactone + NADP+ Rattus norvegicus
-
 D-glucurono-3,6-lactone + NADPH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.20
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.20 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.20 additional information
-
 13450 U/min * mg, 1 unit is defined as change of absorbance of 0.01/min at 340 nm Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.1.1.20 -80°C, 10 days, 30% loss of activity Rattus norvegicus
1.1.1.20 0°C, 2 months, less than 10% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.20 D-galacturonic acid + NADPH
-
 Rattus norvegicus ?
-
 ?
1.1.1.20 D-glucuronic acid + NADPH
-
 Rattus norvegicus L-gulonic acid + NADP+
-
 ?
1.1.1.20 D-glucurono-3,6-lactone + NADPH oxidation activity is very low Rattus norvegicus D-gulono-1,4-lactone + NADP+
-
 r
1.1.1.20 D-iduronic acid + NADPH
-
 Rattus norvegicus ?
-
 ?
1.1.1.20 erythrose + NADPH
-
 Rattus norvegicus glycerol + NADP+
-
 ?
1.1.1.20 glyceraldehyde + NADPH
-
 Rattus norvegicus ?
-
 ?
1.1.1.20 L-gulono-1,4-lactone + NADP+
-
 Rattus norvegicus D-glucurono-3,6-lactone + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.20 monomer 1 * 40000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.20 40
-
 30 min, 20% loss of activity Rattus norvegicus
1.1.1.20 40
-
 60 min, 30% loss of activity Rattus norvegicus
1.1.1.20 40
-
 180 min, 50% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.20 5.5 7.5 DL-glyceraldehyde Rattus norvegicus
1.1.1.20 6 7 D-glucuronic acid Rattus norvegicus
1.1.1.20 9.5
-
 broad, oxidation of glycerol and L-gulonolactone Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.20 NADH NADPH is 145-times as effective as NADH Rattus norvegicus
1.1.1.20 NADP+
-
 Rattus norvegicus
1.1.1.20 NADPH NADPH is 145-times as effective as NADH Rattus norvegicus