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Literature summary extracted from

  • Li, D.; Stevenson, K.J.
    Purification and sequence analysis of a novel NADP(H)-dependent type III alcohol dehydrogenase from Thermococcus strain AN1 (1997), J. Bacteriol., 179, 4433-4437.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.2 gene cloned and sequenced Thermococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.08
-
NADP+
-
Thermococcus sp.
1.1.1.2 0.12 0.13 acetaldehyde
-
Thermococcus sp.
1.1.1.2 1.4
-
butanol
-
Thermococcus sp.
1.1.1.2 1.5
-
NAD+
-
Thermococcus sp.
1.1.1.2 10
-
ethanol
-
Thermococcus sp.
1.1.1.2 12
-
n-Propanol
-
Thermococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 46700
-
4 * 46700 Thermococcus sp.
1.1.1.2 158000
-
gel filtration Thermococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Bacillus methanolicus
-
-
-
1.1.1.2 Clostridium acetobutylicum
-
-
-
1.1.1.2 Drosophila melanogaster
-
-
-
1.1.1.2 Escherichia coli
-
-
-
1.1.1.2 Saccharomyces cerevisiae
-
yeast
-
1.1.1.2 Thermococcus sp.
-
-
-
1.1.1.2 Thermococcus sp. AN1
-
-
-
1.1.1.2 Zymomonas mobilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2
-
Thermococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.2 3.2
-
-
Thermococcus sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.2 4°C half-life 6-7 days when stored aerobically Thermococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 1-octanol + NADP+
-
Thermococcus sp. octanal + NADPH
-
r
1.1.1.2 1-pentanol + NADP+
-
Thermococcus sp. n-pentanal + NADPH
-
r
1.1.1.2 acetaldehyde + NADPH + H+
-
Thermococcus sp. ethanol + NADP+
-
?
1.1.1.2 acetaldehyde + NADPH + H+
-
Thermococcus sp. AN1 ethanol + NADP+
-
?
1.1.1.2 benzyl alcohol + NAD+
-
Thermococcus sp. benzaldehyde + NADH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Escherichia coli acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Saccharomyces cerevisiae acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Zymomonas mobilis acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Clostridium acetobutylicum acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Thermococcus sp. acetaldehyde + NADPH + H+
-
r
1.1.1.2 ethanol + NADP+
-
Thermococcus sp. AN1 acetaldehyde + NADPH + H+
-
r
1.1.1.2 additional information methanol, isopropanol and glycerol are no substrates Thermococcus sp. ?
-
?
1.1.1.2 additional information methanol, isopropanol and glycerol are no substrates Thermococcus sp. AN1 ?
-
?
1.1.1.2 n-butanol + NADP+
-
Zymomonas mobilis butyraldehyde + NADPH
-
r
1.1.1.2 n-butanol + NADP+
-
Thermococcus sp. butyraldehyde + NADPH
-
r
1.1.1.2 n-butanol + NADP+
-
Thermococcus sp. AN1 butyraldehyde + NADPH
-
r
1.1.1.2 n-propanol + NADP+
-
Thermococcus sp. propionaldehyde + NADPH
-
r
1.1.1.2 n-propanol + NADP+
-
Thermococcus sp. AN1 propionaldehyde + NADPH
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.2 tetramer 4 * 46700 Thermococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 85
-
ethanol as substrate Thermococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.2 80
-
half-life of 16 min Thermococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 6.8 7 ethanol as substrate Thermococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADPH
-
Thermococcus sp.