EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.191 | hydroxymethylindole | 42% activation at 0.2 mM | Phycomyces blakesleeanus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.191 | Cu2+ | 59% inhibition at 1 mM | Phycomyces blakesleeanus | |
1.1.1.191 | Hg2+ | 50% inhibition at 0.01 mM | Phycomyces blakesleeanus | |
1.1.1.191 | Indole-3-aldehyde | slight inhibition at 0.2 mM | Phycomyces blakesleeanus | |
1.1.1.191 | indole-3-glycolate | 62% inhibition at 0.2 mM | Phycomyces blakesleeanus | |
1.1.1.191 | KI | 23% inhibition at 1 mM | Phycomyces blakesleeanus | |
1.1.1.191 | Mn2+ | 46% inhibition at 1 mM | Phycomyces blakesleeanus | |
1.1.1.191 | NADP+ | non-competitive inhibition, half-maximal inhibition at 0.2 mM | Phycomyces blakesleeanus | |
1.1.1.191 | Zn2+ | 36% inhibition at 1 mM | Phycomyces blakesleeanus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.191 | 0.024 | - |
NADPH | - |
Phycomyces blakesleeanus | |
1.1.1.191 | 0.2 | - |
Indole-3-acetaldehyde | - |
Phycomyces blakesleeanus | |
1.1.1.191 | 0.645 | - |
dihydroxyacetone phosphate | - |
Phycomyces blakesleeanus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.191 | Fe3+ | 22% activation at 0.1 mM | Phycomyces blakesleeanus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.191 | indole-3-acetaldehyde + NADPH | Phycomyces blakesleeanus | - |
indole-3-ethanol + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.191 | Phycomyces blakesleeanus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.191 | partial | Phycomyces blakesleeanus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.191 | cell culture | maximum enzyme activity from cultures after 48 h | Phycomyces blakesleeanus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.191 | 0.06 | - |
light-grown cells | Phycomyces blakesleeanus |
1.1.1.191 | 0.11 | - |
dark-grown cells | Phycomyces blakesleeanus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.191 | acetaldehyde + NADPH + H+ | very low activity | Phycomyces blakesleeanus | ethanol + NADP+ | - |
? | |
1.1.1.191 | benzaldehyde + NADPH | 2.5fold higher activity than for indole-3-acetaldehyde | Phycomyces blakesleeanus | benzyl alcohol + NADP+ | - |
? | |
1.1.1.191 | dihydroxyacetonephosphate + NADPH | well accepted substrate | Phycomyces blakesleeanus | glycerol 1-phosphate + NADP+ | - |
? | |
1.1.1.191 | indole-3-acetaldehyde + NADPH | - |
Phycomyces blakesleeanus | indole-3-ethanol + NADP+ | - |
? | |
1.1.1.191 | indole-3-aldehyde + NADPH | 19% of the activity of indole-3-acetaldehyde | Phycomyces blakesleeanus | indole-3-ol + NADP+ | - |
? | |
1.1.1.191 | phenylacetaldehyde + NADPH + H+ | well accepted substrate | Phycomyces blakesleeanus | phenylethanol + NADP+ | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.191 | 30 | - |
- |
Phycomyces blakesleeanus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.191 | 6 | 8 | - |
Phycomyces blakesleeanus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.191 | NADP+ | with NAD+ 10times lower activity | Phycomyces blakesleeanus |