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Literature summary extracted from
- Purification of NADPH-specific indole-3-acetaldehyde reductase from Cucumis sativus by two-dimensional native polyacrylamide gel electrophoresis (1989), Physiol. Plant., 77, 613-619.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.191 | dihydroxyacetonephosphate | non-competitive inhibition, Ki: 0.35 mM | Cucumis sativus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.191 | 0.025 | - |
Indole-3-acetaldehyde | - |
Cucumis sativus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.191 | indole-3-acetaldehyde + NADPH | Cucumis sativus | substrates hold key positions in the regulation of indole-3-acetic acid content in higher plants | indole-3-ethanol + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.191 | Cucumis sativus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.191 | to homogeneity, chromatography steps, native PAGE | Cucumis sativus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.191 | fruit | - |
Cucumis sativus | - |
| 1.1.1.191 | seed | - |
Cucumis sativus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.191 | 0.002 | - |
partial purified from seedlings | Cucumis sativus |
| 1.1.1.191 | 0.014 | - |
partial purified from fruits | Cucumis sativus |
| 1.1.1.191 | 0.04 | - |
cytosolic fraction | Cucumis sativus |
| 1.1.1.191 | 0.24 | - |
purified from fruits | Cucumis sativus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.191 | indole-3-acetaldehyde + NADPH | substrates hold key positions in the regulation of indole-3-acetic acid content in higher plants | Cucumis sativus | indole-3-ethanol + NADP+ | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.191 | NADP+ | - |
Cucumis sativus | |
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