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Literature summary extracted from

  • Brown, H.M.; Purves, W.K.
    Isolation and characterization of indole-3-acetaldehyde reductases from Cucumis sativus (1976), J. Biol. Chem., 251, 907-913.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.191 NaCl activation up to 0.1 M Cucumis sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.190 Ionic strength slight inhibition by increasing ionic strength Cucumis sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.190 0.18
-
 NADH
-
 Cucumis sativus
1.1.1.190 0.4
-
 Indole-3-acetaldehyde
-
 Cucumis sativus
1.1.1.191 0.067
-
 Indole-3-acetaldehyde at pH 7.0, isoenzyme TH Cucumis sativus
1.1.1.191 0.133
-
 Indole-3-acetaldehyde at pH 7.0, isoenzyme TL Cucumis sativus
1.1.1.191 0.2
-
 Indole-3-acetaldehyde at pH 5.2, isoenzyme TH Cucumis sativus
1.1.1.191 0.25
-
 Indole-3-acetaldehyde at pH 5.2, isoenzyme TL Cucumis sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.190 33000
-
 gel filtration Cucumis sativus
1.1.1.191 17000
-
 isoenzyme TL, gel filtration Cucumis sativus
1.1.1.191 52000
-
 isoenzyme TH, gel filtration Cucumis sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.190 indole-3-acetaldehyde + NADH Cucumis sativus strong substrate specificity indole-3-ethanol + NAD+
-
 ir
1.1.1.191 indole-3-acetaldehyde + NADPH Cucumis sativus
-
 indole-3-ethanol + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.190 Cucumis sativus
-
-
-
1.1.1.191 Cucumis sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.190 partial Cucumis sativus
1.1.1.191 partial Cucumis sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.190 seedling
-
 Cucumis sativus
-
1.1.1.191 seed two isoenzymes, TH and TL Cucumis sativus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.190 indole-3-acetaldehyde + NADH strong substrate specificity Cucumis sativus indole-3-ethanol + NAD+
-
 ir
1.1.1.190 phenylacetaldehyde + NADH + H+ low activity Cucumis sativus phenylethanol + NAD+
-
 ?
1.1.1.191 acetaldehyde + NADPH + H+ very low activity only for isoenzyme TH Cucumis sativus ethanol + NADP+
-
 ?
1.1.1.191 butyraldehyde + NADPH low activity Cucumis sativus butanol + NADP+
-
 ?
1.1.1.191 indole-3-acetaldehyde + NADPH
-
 Cucumis sativus indole-3-ethanol + NADP+
-
 ir
1.1.1.191 phenylacetone + NADPH low activity Cucumis sativus phenylethanol + NADP+
-
 ?
1.1.1.191 propionaldehyde + NADPH very low activity only for isoenzyme TL Cucumis sativus propanol + NADP+
-
 ?
1.1.1.191 trans-cinnamaldehyde + NADPH very low activity Cucumis sativus (2E)-3-phenylprop-2-en-1-ol + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.190 6.1
-
 second optimum at pH 7 Cucumis sativus
1.1.1.190 7
-
 second optimum at pH 6.1 Cucumis sativus
1.1.1.191 5.2
-
 second pH optimum at pH 7.0 Cucumis sativus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.190 NAD+
-
 Cucumis sativus
1.1.1.191 NADP+
-
 Cucumis sativus