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Literature summary extracted from
- TPN-L-gulonate dehydrogenase (1961), Biochim. Biophys. Acta, 47, 298-306.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.19 | EDTA 1 mM protects against heat denaturation at 40°C | Sus scrofa |
| 1.1.1.19 | lyophilization destroys activity | Sus scrofa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.19 | p-chloromercuribenzoate | - |
Sus scrofa |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.19 | 0.02 | - |
NADP+ | uncoupled reaction | Sus scrofa | |
| 1.1.1.19 | 7.3 | - |
L-gulonate | - |
Sus scrofa | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.1.1.19 | Acetone | inactivates | Sus scrofa |
| 1.1.1.19 | Ethanol | inactivates | Sus scrofa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.19 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.19 | - |
Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.19 | kidney | - |
Sus scrofa | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.19 | -5°C, 2 weeks | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.19 | D-galacturonate + NADPH | - |
Sus scrofa | ? | - |
r | |
| 1.1.1.19 | L-gulonate + NADP+ | - |
Sus scrofa | D-glucuronate + NADPH | - |
r | |
| 1.1.1.19 | additional information | no reaction with gamma-lactones of the substrates | Sus scrofa | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.19 | 40 | - |
15 min, 50% loss of activity, ammonium sulfate fraction | Sus scrofa |
| 1.1.1.19 | 50 | - |
15 min, complete inactivation, ammonium sulfate fraction | Sus scrofa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.19 | 8.6 | 9.3 | - |
Sus scrofa |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.19 | 7 | - |
most stable | Sus scrofa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.19 | NADP+ | - |
Sus scrofa | |
| 1.1.1.19 | NADPH | - |
Sus scrofa | |
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Release 2023.1 (January 2023)
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