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Literature summary extracted from

  • Yamanaka, K.; Gino, M.; Kaneda, R.
    A specific NAD-D-xylose dehydrogenase from Arthrobacter sp. (1977), Agric. Biol. Chem., 41, 1493-1499.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.175 17.4
-
D-xylose
-
Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.175 62000
-
gel filtration Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.175 D-xylose + NAD+ Arthrobacter sp. highly specific for both substrates D-xylonolactone + NADH + H+
-
?
1.1.1.175 D-xylose + NAD+ Arthrobacter sp. 588 highly specific for both substrates D-xylonolactone + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.175 Arthrobacter sp.
-
-
-
1.1.1.175 Arthrobacter sp. 588
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.175 to homogeneity, chromatography techniques Arthrobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.175 culture condition:D-xylose-grown cell inducible enzyme synthesis by growth in D-xylose-containing medium Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.175 24.12
-
-
Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.175 D-xylose + NAD+ highly specific for both substrates Arthrobacter sp. D-xylonolactone + NADH + H+
-
?
1.1.1.175 D-xylose + NAD+ highly specific for both substrates Arthrobacter sp. 588 D-xylonolactone + NADH + H+
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.175 30 35
-
Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.175 30
-
stable below Arthrobacter sp.
1.1.1.175 50
-
complete inactivation Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.175 10.4
-
-
Arthrobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.175 6.5 7.8 stable within, no enzymatic activity Arthrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.175 NAD+ cannot be replaced by NADP+ Arthrobacter sp.