Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yamanaka, K.; Gino, M.
    Purification and properties of D-xylose dehydrogenase in bacteria (1979), Hakko Kogaku Kaishi, 57, 322-331.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.175 0.35
-
 NAD+ strain 89B Pseudomonas sp.
1.1.1.175 0.39
-
 NAD+ strain 90A Pseudomonas sp.
1.1.1.175 0.77
-
 D-xylose strain 90A Pseudomonas sp.
1.1.1.175 2.9
-
 D-xylose strain 89B Pseudomonas sp.
1.1.1.175 5.9
-
 L-arabinose strain 89B Pseudomonas sp.
1.1.1.175 30
-
 D-galactose strain 89B Pseudomonas sp.
1.1.1.175 100
-
 L-arabinose strain 90A Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.175 67000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.175 D-galactose + NAD+ Pseudomonas sp. low activity ? + NADH
-
 ?
1.1.1.175 D-glucose + NAD+ Pseudomonas sp.
-
 ? + NADH
-
 ?
1.1.1.175 D-lyxose + NAD+ Pseudomonas sp. low activity ? + NADH
-
 ?
1.1.1.175 D-xylose + NAD+ Pseudomonas sp.
-
 D-xylonolactone + NADH + H+
-
 ?
1.1.1.175 L-arabinose + NAD+ Pseudomonas sp.
-
 ? + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.175 Pseudomonas sp.
-
 strains 89B, 90A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.175 to homogeneity, chromatography techniques, isoelectric focusing Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.175 culture condition:D-xylose-grown cell inducible enzyme synthesis by growth in D-xylose-containing medium Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.175 30.9
-
 strain 89B Pseudomonas sp.
1.1.1.175 143
-
 strain 90A Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.175 D-galactose + NAD+ low activity Pseudomonas sp. ? + NADH
-
 ?
1.1.1.175 D-glucose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
1.1.1.175 D-lyxose + NAD+ low activity Pseudomonas sp. ? + NADH
-
 ?
1.1.1.175 D-xylose + NAD+
-
 Pseudomonas sp. D-xylonolactone + NADH + H+
-
 ?
1.1.1.175 L-arabinose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.175 40 55
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.175 9 10
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.175 5.3 9 stable within, strain 89B Pseudomonas sp.
1.1.1.175 6 7.5 stable within, strain 90A Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.175 NAD+
-
 Pseudomonas sp.