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Literature summary extracted from

  • Matak-Vinkovic, D.; Vinkovic, M.; Saldanha, S.A.; Ashurst, J.L.; von Delft, F.; Inoue, T.; Miguel, R.N.; Smith, A.G.; Blundell, T.L.; Abell, C.
    Crystal structure of Escherichia coli ketopantoate reductase at 1.7 A resolution and insight into the enzyme mechanism (2001), Biochemistry, 40, 14493-14500.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.169 expression in Escherichia coli BL21(DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.169 prismic crystals, hanging drop vapor-diffusion technique Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.169 additional information Lys176 and Glu256 important for binding ketopantoate and the catalytic mechanism Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.169 0.004
-
 NADPH
-
 Escherichia coli
1.1.1.169 0.02
-
 NADPH
-
 Escherichia coli
1.1.1.169 0.06
-
 ketopantoate
-
 Escherichia coli
1.1.1.169 0.12
-
 ketopantoate
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.169 additional information
-
 Stenotrophomonas maltophilia
1.1.1.169 additional information does not require Mg2+, independent of divalent metal ions Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.169 30500
-
 3-5 * 30500 or 30000, SDS-PAGE Stenotrophomonas maltophilia
1.1.1.169 31100
-
 1 * 31100, HPLC gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.169 33000
-
 SDS-PAGE Escherichia coli
1.1.1.169 34000
-
 1 * 34000 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.169 ketopantoate + NADPH Salmonella enterica subsp. enterica serovar Typhimurium
-
 pantoate + NADP+
-
 ?
1.1.1.169 ketopantoate + NADPH Escherichia coli pantothenate biosynthetic pathway pantoate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.169 Escherichia coli P0A9J4 expressed in BL21(DE3)
-
1.1.1.169 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
1.1.1.169 Stenotrophomonas maltophilia
-
 845
-
1.1.1.169 Stenotrophomonas maltophilia 845
-
 845
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.169 side-chain modification SeMet substitution Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.169 FPLC, anion exchange chromatography, gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.169 additional information
-
-
 Escherichia coli
1.1.1.169 additional information
-
 3.8-fold higher specific activity with NADPH than with NADH Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.169 ketopantoate + NADPH
-
 Salmonella enterica subsp. enterica serovar Typhimurium pantoate + NADP+
-
 ?
1.1.1.169 ketopantoate + NADPH pantothenate biosynthetic pathway Escherichia coli pantoate + NADP+
-
 ?
1.1.1.169 additional information
-
 Escherichia coli ?
-
 ?
1.1.1.169 additional information 3.8-fold higher specific activity with NADPH than with NADH Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.169 monomer 1 * 31100, HPLC gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.169 monomer 1 * 34000 Escherichia coli
1.1.1.169 monomer 1 * 33000, gel filtration Escherichia coli
1.1.1.169 oligomer 3-5 * 30500 or 30000, SDS-PAGE Stenotrophomonas maltophilia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.169 8
-
 ketopantoate
-
 Escherichia coli
1.1.1.169 40
-
 ketopantoate
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.169 NADPH
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.169 NADPH
-
 Stenotrophomonas maltophilia
1.1.1.169 NADPH
-
 Escherichia coli