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Literature summary extracted from

  • Wilken, D.R.; King, H.L.; Dyar, R.E.
    Ketopantoic acid and ketopantoyl lactone reductases. Stereospecificity of transfer of hydrogen from reduced nicotinamide adenine dinucleotide phosphate (1975), J. Biol. Chem., 250, 2311-2314.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.168 2-dehydropantoyl lactone + NADPH Saccharomyces cerevisiae
-
 (R)-pantoyl lactone + NADP+
-
 ?
1.1.1.214 additional information Escherichia coli the physiological substrate for the enzyme is uncertain, the physiological function of the enzyme is unknown ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.168 Saccharomyces cerevisiae
-
-
-
1.1.1.169 Escherichia coli
-
 ATCC 9637
-
1.1.1.169 Saccharomyces cerevisiae
-
-
-
1.1.1.214 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.169 gel chromatography Escherichia coli
1.1.1.169 gel chromatography Saccharomyces cerevisiae
1.1.1.214 form A and form B of the enzyme obtained separated by rupture, centrifugation and column chromatography on DEAE-cellulose and hydroxylapatite Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.168 2-dehydropantoyl lactone + NADPH
-
 Saccharomyces cerevisiae (R)-pantoyl lactone + NADP+
-
 ?
1.1.1.168 additional information keto-omega-methylpantoyl lactone is the only analog of ketopantoyl lactone tested that is a substrate Saccharomyces cerevisiae additional information
-
 ?
1.1.1.168 additional information not: several other 2-keto acids Saccharomyces cerevisiae additional information
-
 ?
1.1.1.168 additional information not: ketopantoic acid Saccharomyces cerevisiae additional information
-
 ?
1.1.1.169 ketopantoic acid + NADPH B-specific Escherichia coli pantoic acid + NADP+
-
 ?
1.1.1.169 ketopantoic acid + NADPH B-specific Saccharomyces cerevisiae pantoic acid + NADP+
-
 ?
1.1.1.214 keto-omega-methylpantoyl lactone + NADPH the only compound other than ketopantoyl lactone which is a substrate, 73% relative activity to ketopantoyl lactone with form A enzyme, 56% relative activity to ketopantoyl lactone with form B enzyme Escherichia coli methylpantoyl lactone + NADP+
-
 ?
1.1.1.214 ketopantoyl lactone + NADPH
-
 Escherichia coli pantoyl lactone + NADP+
-
 ?
1.1.1.214 additional information both forms of the enzyme are B-specific, the enzyme exhibits opposite stereospecificity from that observed with the Saccharomyces cerevisiae enzyme, which is A-specific, less than 5% relative activity to ketopantoyl lactone with the following 2-keto-gamma-lactones: 2-keto-4-hydroxy-3-methylbutyric acid-gamma-lactone, 2-keto-4-hydroxybutyric acid-gamma-lactone Escherichia coli ?
-
 ?
1.1.1.214 additional information the physiological substrate for the enzyme is uncertain, the physiological function of the enzyme is unknown Escherichia coli ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.169 NADPH B-specific hydrogen transfer Escherichia coli
1.1.1.169 NADPH B-specific hydrogen transfer Saccharomyces cerevisiae
1.1.1.214 additional information no activity observed with NADH Escherichia coli
1.1.1.214 NADPH
-
 Escherichia coli