Literature summary extracted from

Yoshimoto, T.; Higashi, H.; Kanatani, A.; Lin, X.S.; Nagai, H.; Oyama, H.; Kurazono, K.; Tsuru, D.
Cloning and sequencing of the 7alpha-hydroxysteroid dehydrogenase gene from Escherichia coli HB101 and characterization of the expressed enzyme (1991), J. Bacteriol., 173, 2173-2179.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.159	gene from strain HB101 cloned in plasmid pSD1 and expressed in Escherichia coli strain DH1	Escherichia coli

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.1.1.159	-	Escherichia coli

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.1.1.159	CoCl2	nearly complete inhibition	Escherichia coli
1.1.1.159	CuCl2	nearly complete inhibition	Escherichia coli
1.1.1.159	diethyl dicarbonate	complete	Escherichia coli
1.1.1.159	FeCl2	-	Escherichia coli
1.1.1.159	FeCl3	nearly complete inhibition	Escherichia coli
1.1.1.159	HgCl2	-	Escherichia coli
1.1.1.159	iodoacetic acid	70% remaining activity at concentration 1 mM	Escherichia coli
1.1.1.159	N-bromosuccinimide	complete	Escherichia coli
1.1.1.159	NiSO4	-	Escherichia coli
1.1.1.159	o-phenanthroline	70% remaining acitvity at concentration 1 mM	Escherichia coli
1.1.1.159	p-chloromercuribenzoate	80% remaining activity at concentration 0.3 mM	Escherichia coli
1.1.1.159	ZnCl2	-	Escherichia coli

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1.1.159	0.19	-	taurochenodeoxycholic acid	-	Escherichia coli
1.1.1.159	0.43	-	chenodeoxycholic acid	-	Escherichia coli
1.1.1.159	1.2	-	cholic acid	-	Escherichia coli
1.1.1.159	1.25	-	glycocholic acid	-	Escherichia coli
1.1.1.159	2	-	taurocholic acid	-	Escherichia coli

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.1.159	28000	-	4 * 28000, SDS-PAGE	Escherichia coli
1.1.1.159	120000	-	gel filtration	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.159	Escherichia coli	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.159	recombinant from Escherichia coli, high yield	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.159	chenodeoxycholic acid + NAD(P)+	-	Escherichia coli	3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H	-	?
1.1.1.159	cholic acid + NAD(P)+	-	Escherichia coli	3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH	-	?
1.1.1.159	glycocholic acid + NAD+	-	Escherichia coli	N-[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]glycine + NADH	-	?
1.1.1.159	additional information	no activity with deoxycholic acid and taurodeoxycholic acid	Escherichia coli	?	-	?
1.1.1.159	taurochenodeoxycholic acid + NAD+	-	Escherichia coli	2[[3alpha-hydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH	-	?
1.1.1.159	taurocholic acid + NAD+	-	Escherichia coli	2[[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.1.1.159	tetramer	4 * 28000, SDS-PAGE	Escherichia coli

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.1.1.159	0.417	-	glycocholic acid	-	Escherichia coli
1.1.1.159	3.13	-	taurocholic acid	-	Escherichia coli
1.1.1.159	3.43	-	taurochenodeoxycholic acid	-	Escherichia coli
1.1.1.159	8.18	-	chenodeoxycholic acid	-	Escherichia coli
1.1.1.159	63.3	-	cholic acid	-	Escherichia coli

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.159	8.5	-	-	Escherichia coli

pH Stability

EC Number	pH Stability	pH Stability Maximum	Comment	Organism
1.1.1.159	8	9	-	Escherichia coli

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.159	NAD+	-	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)