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Literature summary extracted from

  • Tanabe, T.; Tanaka, N.; Uchikawa, K.; Kabashima, T.; Ito, K.; Nonaka, T.; Mitsui, Y.; Tsuru, M.; Yoshimoto, T.
    Roles of the Ser146, Tyr159, and Lys163 residues in the catalytic action of 7alpha-hydroxysteroid dehydrogenase from Escherichia coli (1998), J. Biochem., 124, 634-641.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.159 site directed mutagenesis Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.159
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.159 K163I 5.25% activity of wild-type activity Escherichia coli
1.1.1.159 K163R 63.7% activity of wild-type activity Escherichia coli
1.1.1.159 S146A 20.3% activity of wild-type activity Escherichia coli
1.1.1.159 S146H 35.6% activity of wild-type activity Escherichia coli
1.1.1.159 Y159F no activity Escherichia coli
1.1.1.159 Y159H 13.3% activity of wild-type activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.159 0.159
-
 NAD+ mutant K163R Escherichia coli
1.1.1.159 0.223
-
 cholic acid mutant K163I Escherichia coli
1.1.1.159 0.279
-
 NAD+
-
 Escherichia coli
1.1.1.159 0.283
-
 cholic acid mutant S146H Escherichia coli
1.1.1.159 0.302
-
 NAD+ mutant S146A Escherichia coli
1.1.1.159 0.329
-
 NAD+ mutant K163I Escherichia coli
1.1.1.159 0.361
-
 cholic acid
-
 Escherichia coli
1.1.1.159 0.364
-
 NAD+ mutant S146H Escherichia coli
1.1.1.159 0.386
-
 NAD+ mutant Y159H Escherichia coli
1.1.1.159 0.407
-
 cholic acid mutant Y159H Escherichia coli
1.1.1.159 0.415
-
 cholic acid mutant S146A Escherichia coli
1.1.1.159 0.612
-
 cholic acid mutant K163R Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.159 28000
-
 4 * 28000, SDS-PAGE Escherichia coli
1.1.1.159 107100
-
 sequence determination Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.159 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.159
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.159 cholate + NAD+ = 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oate + NADH + H+ Tyr159 acts as basic catalyst, Lys163 binds to NAD(H) and lowers the pKa value of Tyr159, Ser146 stabilizes the substrate, reaction intermediate and product in catalysis Escherichia coli
a

Subunits

EC Number Subunits Comment Organism
1.1.1.159 tetramer 4 * 28000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.159 7.92
-
 cholic acid mutant K163I Escherichia coli
1.1.1.159 20.1
-
 cholic acid mutant Y159H Escherichia coli
1.1.1.159 30.7
-
 cholic acid mutant S146A Escherichia coli
1.1.1.159 53.7
-
 cholic acid mutant S146H Escherichia coli
1.1.1.159 96.2
-
 cholic acid mutant K163R Escherichia coli
1.1.1.159 151
-
 cholic acid
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.159 10
-
 oxidation of cholic acid Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.159 6 10
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.159 NAD+ wild type and mutants Escherichia coli