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Literature summary extracted from

  • Baron, S.F.; Franklund, C.V.; Hylemon, P.B.
    Cloning, sequencing, and expression of the gene coding for bile acid 7alpha-hydroxysteroid dehydrogenase from Eubacterium sp. strain VPI 12708 (1991), J. Bacteriol., 173, 4558-4569.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.159 overexpression in Escherichia coli DH5alpha, amino acid sequence analysis and comparison with other dehydrogenases Eubacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.159 110000
-
 wild type and recombinant proteins, non-denaturing PAGE Eubacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.159 bile acids + NAD(P)+ Eubacterium sp.
-
 ?
-
 r
1.1.1.159 bile acids + NAD(P)+ Eubacterium sp. VPI 12708
-
 ?
-
 r
1.1.1.159 additional information Eubacterium sp. cholic acid inducible ?
-
 ?
1.1.1.159 additional information Eubacterium sp. VPI 12708 cholic acid inducible ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.159 Eubacterium sp.
-
-
-
1.1.1.159 Eubacterium sp. VPI 12708
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.159 wild-type and recombinant from Escherichia coli Eubacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.159 332
-
 NADP+, recombinant enzyme from Escherichia coli Eubacterium sp.
1.1.1.159 374
-
 NADP+ Eubacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.159 bile acids + NAD(P)+
-
 Eubacterium sp. ?
-
 r
1.1.1.159 bile acids + NAD(P)+
-
 Eubacterium sp. VPI 12708 ?
-
 r
1.1.1.159 cholic acid + NAD(P)+ inducible by cholic acid Eubacterium sp. 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 r
1.1.1.159 cholic acid + NAD(P)+ inducible by cholic acid Eubacterium sp. VPI 12708 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 r
1.1.1.159 glycochenodeoxycholic acid + NAD(P)+
-
 Eubacterium sp. N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H
-
 r
1.1.1.159 glycochenodeoxycholic acid + NAD(P)+
-
 Eubacterium sp. VPI 12708 N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H
-
 r
1.1.1.159 additional information cholic acid inducible Eubacterium sp. ?
-
 ?
1.1.1.159 additional information cholic acid inducible Eubacterium sp. VPI 12708 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.159 tetramer 4 * 32000-33000 Eubacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.159 NAD+ nearly no activity with Eubacterium sp.
1.1.1.159 NADP+
-
 Eubacterium sp.