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Literature summary extracted from

  • MacDonald, I.A.; Williams, C.N.; Mahony, D.E.; Christie, W.M.
    NAD- and NADP-dependent 7alpha-hydroxysteroid dehydrogenases from bacteroides fragilis (1975), Biochim. Biophys. Acta, 384, 12-24.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.159 NAD+-dependent form lyophilized is stable to freezing/thawing, NADP+-dependent form lyophilized is unstable Bacteroides fragilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.159 0.1
-
 3alpha,7alpha-Dihydroxy-5beta-cholanoyl glycine
-
 Bacteroides fragilis
1.1.1.159 0.1
-
 3alpha,7alpha-dihydroxy-5beta-cholanoic acid
-
 Bacteroides fragilis
1.1.1.159 0.1
-
 3alpha,7alpha-dihydroxy-5beta-cholanoyl taurine
-
 Bacteroides fragilis
1.1.1.159 0.32 0.34 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine
-
 Bacteroides fragilis
1.1.1.159 0.32 0.34 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoic acid
-
 Bacteroides fragilis
1.1.1.159 0.32 0.34 3alpha,7alpha,12alpha-trihydroxycholanoyl taurine
-
 Bacteroides fragilis
1.1.1.159 0.45
-
 NAD+ with chenodeoxycholic acid Bacteroides fragilis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.159 Bacteroides fragilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.159 3alpha,7alpha,12alpha-trihydroxycholanoyl taurine + NAD+
-
 Bacteroides fragilis 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanoyl taurine NADH + H+ +
-
 r
1.1.1.159 3alpha,7alpha-dihydroxy-5beta-cholanoyl taurine + NAD+ i.e. chenodeoxycholic acid Bacteroides fragilis 3alpha-hydroxy-7-oxo-5beta-cholanoyl taurine + NADH + H+
-
 r
1.1.1.159 chenodeoxycholic acid + NAD(P)+ i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid Bacteroides fragilis 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 ?
1.1.1.159 glycochenodeoxycholic acid + NAD(P)+
-
 Bacteroides fragilis N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H
-
 ?
1.1.1.159 glycocholic acid + NAD+
-
 Bacteroides fragilis N-[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]glycine + NADH
-
 ?
1.1.1.159 taurochenodeoxycholic acid + NAD+
-
 Bacteroides fragilis 2[[3alpha-hydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH
-
 ?
1.1.1.159 taurocholic acid + NAD+ i.e. 2[[3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholan-24-yl]amino]ethane sulfonic acid Bacteroides fragilis 2[[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.159 7 9 NADP+-dependent activity Bacteroides fragilis
1.1.1.159 9.5 10 NAD+-dependent activity Bacteroides fragilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.159 NAD+
-
 Bacteroides fragilis
1.1.1.159 NADP+
-
 Bacteroides fragilis