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Literature summary extracted from

  • Kinoshita, S.; Kadota, K.; Inoue, T.; Sawada, H.; Taguchi, H.
    Purification and properties of three kinds of alpha-hydroxysteroid dehydrogenase from Brevibacterium fuscum DC33 (1988), J. Ferment. Technol., 66, 145-152.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.159 0.22
-
 cholic acid
-
 Brevibacterium fuscum
1.1.1.159 0.25
-
 NADH with dehydrocholic acid Brevibacterium fuscum
1.1.1.159 0.27
-
 NAD+ with cholic acid Brevibacterium fuscum
1.1.1.159 0.38
-
 12-oxolithocholic acid
-
 Brevibacterium fuscum
1.1.1.159 0.4
-
 NAD+ with 12-ketolithocholic acid Brevibacterium fuscum
1.1.1.159 0.7
-
 Dehydrocholic acid
-
 Brevibacterium fuscum
1.1.1.159 0.99
-
 7,12-Diketolithocholic acid
-
 Brevibacterium fuscum
1.1.1.159 1.3
-
 NADH with 7,12-diketolithocholic acid Brevibacterium fuscum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.159 27000
-
 4 * 27000, SDS-PAGE Brevibacterium fuscum
1.1.1.159 104000
-
 gel filtration Brevibacterium fuscum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.159 Brevibacterium fuscum
-
-
-
1.1.1.176 Brevibacterium fuscum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.159
-
 Brevibacterium fuscum
1.1.1.176
-
 Brevibacterium fuscum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.159 400
-
 7,12-diketolithocholic acid, NADH Brevibacterium fuscum
1.1.1.159 1600
-
 cholic acid, NAD+ Brevibacterium fuscum
1.1.1.159 1700
-
 12-ketolithocholic acid, NAD+ Brevibacterium fuscum
1.1.1.159 2900
-
 dehydrocholic acid, NADH Brevibacterium fuscum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.159 12-ketochenodeoxycholic acid + NAD+
-
 Brevibacterium fuscum 7,12-diketochenodeoxycholic acid + NADH
-
 r
1.1.1.159 12-oxolithocholic acid + NAD+
-
 Brevibacterium fuscum ?
-
 r
1.1.1.159 7,12-diketolithocholic acid + NADH
-
 Brevibacterium fuscum 12-ketochenodeoxycholic acid + NAD+
-
 r
1.1.1.159 chenodeoxycholic acid + NAD(P)+ i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid Brevibacterium fuscum 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 ?
1.1.1.159 cholic acid + NAD(P)+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate Brevibacterium fuscum 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 r
1.1.1.159 dehydrocholic acid + NADH
-
 Brevibacterium fuscum 3,12-diketolithocholic acid + NAD+
-
 r
1.1.1.159 glycochenodeoxycholic acid + NAD(P)+
-
 Brevibacterium fuscum N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H
-
 ?
1.1.1.159 glycocholic acid + NAD+ i.e. N-[3alpha,7alpha,12alpha-trihydroxy-24-oxocholan-24-yl]glycine Brevibacterium fuscum N-[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]glycine + NADH
-
 ?
1.1.1.159 taurocholic acid + NAD+ i.e. 2[[3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholan-24-yl]amino]ethane sulfonic acid Brevibacterium fuscum 2[[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH
-
 ?
1.1.1.176 cholic acid + NAD(P)+
-
 Brevibacterium fuscum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 ?
1.1.1.176 deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid Brevibacterium fuscum 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
 ?
1.1.1.176 glycocholic acid + NAD+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine Brevibacterium fuscum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
 ?
1.1.1.176 glycodeoxycholic acid + NAD+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine Brevibacterium fuscum 3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
 ?
1.1.1.176 taurocholic acid + NAD(P)+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine Brevibacterium fuscum 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H
-
 ?
1.1.1.176 taurodeoxycholic acid + NAD+
-
 Brevibacterium fuscum 3alpha-hydroxy-12-oxo-5beta-cholanoyl taurine + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.159 tetramer 4 * 27000, SDS-PAGE Brevibacterium fuscum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.159 25 30
-
 Brevibacterium fuscum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.159 25 45
-
 Brevibacterium fuscum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.159 30
-
 stable below Brevibacterium fuscum
1.1.1.159 40
-
 inactivation at Brevibacterium fuscum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.159 additional information
-
 value for reduction of dehydrocholic acid below 5.2, not measurable due to insolubility of substrate Brevibacterium fuscum
1.1.1.159 5.3
-
 dehydrocholic acid reduction Brevibacterium fuscum
1.1.1.159 10.5
-
 oxidation of cholic acid Brevibacterium fuscum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.159 5.7 10.5 5°C overnight Brevibacterium fuscum
1.1.1.159 6 11
-
 Brevibacterium fuscum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.159 NAD+
-
 Brevibacterium fuscum