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Literature summary extracted from

  • Prabha, V.; Gupta, M.; Gupta, K.G.
    Kinetic properties of 7alpha-hydroxysteroid dehydrogenase from Escherichia coli 080 (1989), Can. J. Microbiol., 35, 1076-1080.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.159 2-mercaptoethanol activation Escherichia coli
1.1.1.159 dithiothreitol activation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.159 ascorbic acid
-
 Escherichia coli
1.1.1.159 BaCl2
-
 Escherichia coli
1.1.1.159 CoCl2
-
 Escherichia coli
1.1.1.159 CuCl2
-
 Escherichia coli
1.1.1.159 EDTA
-
 Escherichia coli
1.1.1.159 FeCl3
-
 Escherichia coli
1.1.1.159 HgCl2
-
 Escherichia coli
1.1.1.159 MgCl2
-
 Escherichia coli
1.1.1.159 NH4Cl
-
 Escherichia coli
1.1.1.159 potassium oxalate
-
 Escherichia coli
1.1.1.159 Sodium citrate
-
 Escherichia coli
1.1.1.159 sodium lauryl sulfate
-
 Escherichia coli
1.1.1.159 Sodium perchlorate
-
 Escherichia coli
1.1.1.159 Sodium periodate
-
 Escherichia coli
1.1.1.159 Sodium persulfate
-
 Escherichia coli
1.1.1.159 Triton X-100
-
 Escherichia coli
1.1.1.159 Tween
-
 Escherichia coli
1.1.1.159 ZnCl2
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.159 CaCl2 activation Escherichia coli
1.1.1.159 KCl activation Escherichia coli
1.1.1.159 MnCl2 activation Escherichia coli
1.1.1.159 NaCl activation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.159 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.159 45
-
 deoxycholic acid or lithocholic acid Escherichia coli
1.1.1.159 450
-
-
 Escherichia coli
1.1.1.159 2348
-
 taurocholic acid Escherichia coli
1.1.1.159 2916
-
 glycocholic acid Escherichia coli
1.1.1.159 3486
-
 cholic acid Escherichia coli
1.1.1.159 3743
-
 taurochenodeoxycholic acid Escherichia coli
1.1.1.159 3856
-
 glycochenodeoxycholic acid Escherichia coli
1.1.1.159 4164
-
 chenodeoxycholic acid Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.159 chenodeoxycholic acid + NAD(P)+ i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid Escherichia coli 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 ?
1.1.1.159 cholic acid + NAD(P)+
-
 Escherichia coli 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 ?
1.1.1.159 glycochenodeoxycholic acid + NAD(P)+
-
 Escherichia coli N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H
-
 ?
1.1.1.159 glycocholic acid + NAD+ i.e. N-[3alpha,7alpha,12alpha-trihydroxy-24-oxocholan-24-yl]glycine Escherichia coli N-[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]glycine + NADH
-
 ?
1.1.1.159 taurochenodeoxycholic acid + NAD+
-
 Escherichia coli 2[[3alpha-hydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH
-
 ?
1.1.1.159 taurocholic acid + NAD+ i.e. 2[[3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholan-24-yl]amino]ethane sulfonic acid Escherichia coli 2[[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH
-
 ?