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Literature summary extracted from
- Overexpression, purification, and mechanistic study of UDP-N-acetylenolpyruvylglucosamine reductase (1993), Biochemistry, 32, 2024-2030.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.3.1.98 | overexpression in Escherichia coli XA90F'lacIQ1 | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.3.1.98 | S229A | by site-directed mutagenesis | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.98 | 0.0125 | - |
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine | - |
Escherichia coli |  |
| 1.3.1.98 | 0.0199 | - |
NADPH | - |
Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.98 | 37850 | - |
determined by cDNA sequence | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.98 | UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | Escherichia coli | - |
UDP-N-acetyl-3-D-muramate + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.98 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.1.98 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.98 | 31.4 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.98 | UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | - |
Escherichia coli | UDP-N-acetyl-3-D-muramate + NADP+ | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.98 | FAD | tightly bound flavin cofactor FAD | Escherichia coli | |
| 1.3.1.98 | NADPH | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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