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Literature summary extracted from

  • Hillmer, P.; Gottschalk, G.
    Solubilization and partial characterization of particulate dehydrogenases from Clostridium kluyveri (1974), Biochim. Biophys. Acta, 334, 12-23.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.10 0.033
-
 CoA
-
 Clostridium kluyveri
1.2.1.10 0.4
-
 NAD+
-
 Clostridium kluyveri
1.2.1.10 0.45
-
 acetaldehyde
-
 Clostridium kluyveri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.10 particle-bound
-
 Clostridium kluyveri
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+ Clostridium kluyveri
-
 acetoacetyl-CoA + NADH
-
 r
1.2.1.10 acetaldehyde + CoA + NAD+ Clostridium kluyveri
-
 acetyl-CoA + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.35 Clostridium kluyveri
-
-
-
1.2.1.10 Clostridium kluyveri
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.35 0.41
-
 enzyme activity in solubilized sediments of homogenized cells Clostridium kluyveri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+
-
 Clostridium kluyveri acetoacetyl-CoA + NADH
-
 r
1.2.1.10 acetaldehyde + CoA + NAD+
-
 Clostridium kluyveri acetyl-CoA + NADH
-
 ?
1.2.1.10 caprylaldehyde + CoA + NAD+
-
 Clostridium kluyveri caprylyl-CoA + NADH
-
 ?
1.2.1.10 heptylaldehyde + CoA + NAD+
-
 Clostridium kluyveri heptanoyl-CoA + NADH
-
 ?
1.2.1.10 hexylaldehyde + CoA + NAD+
-
 Clostridium kluyveri hexanoyl-CoA + NADH
-
 ?
1.2.1.10 n-butyraldehyde + CoA + NAD+
-
 Clostridium kluyveri n-butyryl-CoA + NADH
-
 r
1.2.1.10 propanal + CoA + NAD+
-
 Clostridium kluyveri propionyl-CoA + NADH
-
 ?
1.2.1.10 valeraldehyde + CoA + NAD+
-
 Clostridium kluyveri valeryl-CoA + NADH
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.10 9
-
 NAD+ reduction Clostridium kluyveri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.10 8.5 10 70% of maximal activity at pH 8.5, 80% of maximal activity at pH 10 Clostridium kluyveri

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.35 NAD+
-
 Clostridium kluyveri
1.2.1.10 CoA
-
 Clostridium kluyveri
1.2.1.10 NAD+
-
 Clostridium kluyveri