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Literature summary extracted from

  • Sliwkowski, M.X.; Hartmanis, M.G.N.
    Simultaneous single-step purification of thiolase and NADP-dependent 3-hydroxybutyryl-CoA dehydrogenase from Clostridium kluyveri (1984), Anal. Biochem., 141, 344-347.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.157 33500
-
 x * 33500, SDS-PAGE Clostridium kluyveri
2.3.1.9 41000
-
 4 * 41000, SDS-PAGE Clostridium kluyveri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.157 3-acetoacetyl-CoA + NADPH + H+ Clostridium kluyveri
-
 (S)-3-hydroxybutanoyl-CoA + NADP+
-
 r
2.3.1.9 CoA + acetoacetyl-CoA Clostridium kluyveri
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.157 Clostridium kluyveri
-
-
-
2.3.1.9 Clostridium kluyveri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.157 simultaneous single-step purification of thiolase and NADP-dependent 3-hydroxybutyryl-CoA dehydrogenase Clostridium kluyveri
2.3.1.9 CM-Sephadex Clostridium kluyveri
2.3.1.9 single-step purification Clostridium kluyveri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.157 292
-
 pH 6.5, 25°C Clostridium kluyveri
2.3.1.9 115
-
-
 Clostridium kluyveri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.157 3-acetoacetyl-CoA + NADPH + H+
-
 Clostridium kluyveri (S)-3-hydroxybutanoyl-CoA + NADP+
-
 r
1.1.1.157 3-acetoacetyl-CoA + NADPH + H+ NADPH-dependent enzyme specific for 3-hydroxybutyryl-CoA Clostridium kluyveri (S)-3-hydroxybutanoyl-CoA + NADP+
-
 r
2.3.1.9 CoA + acetoacetyl-CoA
-
 Clostridium kluyveri acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA
-
 Clostridium kluyveri acetyl-CoA + acetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.157 ? x * 33500, SDS-PAGE Clostridium kluyveri
2.3.1.9 tetramer 4 * 41000, SDS-PAGE Clostridium kluyveri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.157 25
-
 assay at Clostridium kluyveri
2.3.1.9 25
-
 assay at Clostridium kluyveri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.157 6.5
-
 assay at Clostridium kluyveri

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.157 NADPH specific for NADPH Clostridium kluyveri

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.157 Clostridium kluyveri isoelectric focusing, pH 3.5-10.0
-
 7.8