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Literature summary extracted from

  • Cho, S.H.; Na, J.U.; Youn, H.; Hwang, C.S.; Lee, C.H.; Kang, S.O.
    Sepiapterin reductase producing L-threo-dihydrobiopterin from Chlorobium tepidum (1999), Biochem. J., 340, 497-503.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.1.325 stability of the purified enzyme disappears in sodium citrate buffer and potassium phosphate buffer for 24 h at 4°C, it increases continuously in Tris-HCl buffer and in glycine/NaOH buffer Chlorobaculum tepidum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.153 Melatonin very slight Chlorobaculum tepidum
1.1.1.153 N-acetyldopamine very slight Chlorobaculum tepidum
1.1.1.153 N-acetylserotonin very slight Chlorobaculum tepidum
1.1.1.325 additional information N-acetylserotonin and melatonin are no inhibitors Chlorobaculum tepidum
1.1.1.325 N-acetyldopamine
-
 Chlorobaculum tepidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.153 0.0062
-
 NADPH
-
 Chlorobaculum tepidum
1.1.1.153 0.021
-
 sepiapterin
-
 Chlorobaculum tepidum
1.1.1.153 4
-
 diacetyl
-
 Chlorobaculum tepidum
1.1.1.325 0.0062
-
 NADPH pH 8.8, 45°C Chlorobaculum tepidum
1.1.1.325 0.021
-
 sepiapterin pH 8.8, 45°C Chlorobaculum tepidum
1.1.1.325 4
-
 diacetyl pH 8.8, 45°C Chlorobaculum tepidum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.325 cytosol
-
 Chlorobaculum tepidum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.153 26000
-
 2 * 26000, gradient SDS-PAGE Chlorobaculum tepidum
1.1.1.153 55000
-
 gel filtration Chlorobaculum tepidum
1.1.1.325 26000
-
 2 * 26000, SDS-PAGE Chlorobaculum tepidum
1.1.1.325 55000
-
 gel filtration Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH Chlorobaculum tepidum
-
 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?
1.1.1.325 6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH + H+ Chlorobaculum tepidum
-
 L-threo-7,8-dihydrobiopterin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.153 Chlorobaculum tepidum
-
-
-
1.1.1.325 Chlorobaculum tepidum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.153
-
 Chlorobaculum tepidum
1.1.1.325 native enzyme 533fold from cytosol by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration followed by affinity chromatography, another different step of anion exchange chromatography, and ultrafiltration Chlorobaculum tepidum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.153 L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+ enzyme also reduces carbonyl compounds of non-pteridine derivatives Chlorobaculum tepidum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.153 31.5
-
-
 Chlorobaculum tepidum

Storage Stability

EC Number Storage Stability Organism
1.1.1.153 -70°C, in 20 mM Tris-HCl, pH 8.8 Chlorobaculum tepidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH
-
 Chlorobaculum tepidum 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?
1.1.1.153 diacetyl + NADPH
-
 Chlorobaculum tepidum ?
-
 ?
1.1.1.153 dihydrotepidopterin + NADP+ 15% relative activity Chlorobaculum tepidum ?
-
 ?
1.1.1.153 L-erythro-dihydrobiopterin + NADP+
-
 Chlorobaculum tepidum ?
-
 ?
1.1.1.153 L-threo-dihydrobiopterin + NADP+ D-isomer 45% relative activity Chlorobaculum tepidum ?
-
 r
1.1.1.153 L-threo-dihydroneopterin + NADP+
-
 Chlorobaculum tepidum ?
-
 ?
1.1.1.153 additional information not: methylglyoxal, menadione Chlorobaculum tepidum ?
-
 ?
1.1.1.153 phenylglyoxal + NADPH
-
 Chlorobaculum tepidum ?
-
 ?
1.1.1.325 6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH + H+
-
 Chlorobaculum tepidum L-threo-7,8-dihydrobiopterin + NADP+
-
 ?
1.1.1.325 diacetyl + NADPH + H+
-
 Chlorobaculum tepidum ? + NADP+
-
 ?
1.1.1.325 L-threo-7,8-dihydrobiopterin + NADP+
-
 Chlorobaculum tepidum sepiapterin + NADPH + H+
-
 r
1.1.1.325 additional information the enzyme also shows activity with D-threo-dihydrobiopterin, L-threo-dihydroneopterin, and dihydrotepidopterin, and low activity with L-erythro-dihydrobiopterin Chlorobaculum tepidum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.153 homodimer 2 * 26000, gradient SDS-PAGE Chlorobaculum tepidum
1.1.1.325 homodimer 2 * 26000, SDS-PAGE Chlorobaculum tepidum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.325 sepiapterin reductase
-
 Chlorobaculum tepidum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.153 50
-
 highest activity, activity decreases slowly above Chlorobaculum tepidum
1.1.1.325 50
-
-
 Chlorobaculum tepidum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.153 25 50 relativley stable for 2 h in this range, loss of activity in 20 min at 70°C Chlorobaculum tepidum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.325 5
-
 NADPH pH 8.8, 45°C Chlorobaculum tepidum
1.1.1.325 5
-
 sepiapterin pH 8.8, 45°C Chlorobaculum tepidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.153 8.8
-
 in Tris-HCl buffer or in glycine-NaOH buffer Chlorobaculum tepidum
1.1.1.325 8.8
-
-
 Chlorobaculum tepidum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.153 additional information does not show any activity with NADH Chlorobaculum tepidum
1.1.1.325 NADPH
-
 Chlorobaculum tepidum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.325 0.4
-
 N-acetyldopamine pH 8.8, 45°C Chlorobaculum tepidum

General Information

EC Number General Information Comment Organism
1.1.1.325 additional information alignment of amino acid sequences near the N-termini of sepiapterin reductases from various sources, overview Chlorobaculum tepidum