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Literature summary extracted from

  • Smith, G.K.
    On the role of sepiapterin reductase in the biosynthesis of tetrahydrobiopterin (1987), Arch. Biochem. Biophys., 255, 254-266.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.153 additional information inhibition by specific antibody from rabbits Rattus norvegicus
1.1.1.153 N-acetylserotonin
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.153 additional information
-
 additional information kinetics Rattus norvegicus
1.1.1.153 0.002
-
 pyruvoyltetrahydropterin pH 7.4, 37°C Rattus norvegicus
1.1.1.153 0.007
-
 1'-hydroxy-2'-oxopropyltetrahydropterin pH 7.4, 37°C Rattus norvegicus
1.1.1.153 0.008
-
 lactoyltetrahydropterin pH 7.4, 37°C Rattus norvegicus
1.1.1.153 0.009
-
 sepiapterin pH 7.4, 37°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.153 27500
-
 2 * 27500, SDS-PAGE Rattus norvegicus
1.1.1.153 56000
-
 second band in SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH Rattus norvegicus terminal step in the biosynthetic pathway for tetrahydrobiopterin 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH Rattus norvegicus catalyzes the complete reduction of pyrovoyltetrahydropterin to tetrahydrobiopterin 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.153 Rattus norvegicus
-
-
-
1.1.1.153 Rattus norvegicus
-
 male sprague-dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.153
-
 Rattus norvegicus
1.1.1.153 native enzyme from erythrocytes by hydroxylapatite and NADP+-affinity chromatography, to homogeneity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.153 adrenal gland
-
 Rattus norvegicus
-
1.1.1.153 blood
-
 Rattus norvegicus
-
1.1.1.153 brain
-
 Rattus norvegicus
-
1.1.1.153 erythrocyte
-
 Rattus norvegicus
-
1.1.1.153 heart
-
 Rattus norvegicus
-
1.1.1.153 kidney
-
 Rattus norvegicus
-
1.1.1.153 liver
-
 Rattus norvegicus
-
1.1.1.153 lung
-
 Rattus norvegicus
-
1.1.1.153 spleen
-
 Rattus norvegicus
-
1.1.1.153 testis
-
 Rattus norvegicus
-
1.1.1.153 thymus
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.153 1'-hydroxy-2'-oxopropyltetrahydropterin + NADP+
-
 Rattus norvegicus L-erythro-tetrahydrobiopterin + NADPH
-
 ?
1.1.1.153 1'-hydroxy-2'-oxopropyltetrahydropterin + NADPH
-
 Rattus norvegicus L-erythrotetrahydrobiopterin + NADP+
-
 ?
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH
-
 Rattus norvegicus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH terminal step in the biosynthetic pathway for tetrahydrobiopterin Rattus norvegicus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH catalyzes the complete reduction of pyrovoyltetrahydropterin to tetrahydrobiopterin Rattus norvegicus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?
1.1.1.153 6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
-
 Rattus norvegicus L-erythro-tetrahydrobiopterin + 2 NADP+
-
 ?
1.1.1.153 lactoyltetrahydropterin + NADPH
-
 Rattus norvegicus L-erythrotetrahydrobiopterin + NADP+
-
 ?
1.1.1.153 lactoyltetrahydropterin + NADPH + H+
-
 Rattus norvegicus L-erythro-tetrahydrobiopterin + NADP+
-
 ?
1.1.1.153 additional information descending substrate preference: sepiapterin, lactoyltetrahydropterin, pyruvoyltetrahydropterin, 1'-hydroxy-2'-oxopropyltetrahydropterin Rattus norvegicus ?
-
 ?
1.1.1.153 pyruvoyltetrahydropterin + NADPH
-
 Rattus norvegicus L-erythrotetrahydrobiopterin + NADP+
-
 ?
1.1.1.153 sepiapterin + NADPH + H+ best substrate Rattus norvegicus dihydrobiopterin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.153 ? x * 56000, SDS-PAGE Rattus norvegicus
1.1.1.153 homodimer 2 * 27500, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.153 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.153 7.4
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.153 NADPH
-
 Rattus norvegicus