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Literature summary extracted from

  • Citron, B.A.; Milstien, S.; Gutierrez, J.C.; Levine, R.A.; Yanak, B.L.; Kaufman, S.
    Isolation and expression of rat liver sepiapterin reductase cDNA (1990), Proc. Natl. Acad. Sci. USA, 87, 6436-6440.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.153 recombinant protein expressed in Escherichia coli Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH Rattus norvegicus terminal step in the biosynthetic pathway for tetrahydrobiopterin 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.153 Rattus norvegicus
-
 expressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.153 liver Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.153 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.153 1.7
-
 in Escherichia coli HB101, transformed with pBLY1 containing sepiapterin reductase cDNA Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH
-
 Rattus norvegicus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH terminal step in the biosynthetic pathway for tetrahydrobiopterin Rattus norvegicus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.153 NADPH
-
 Rattus norvegicus