Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hara, A.; Inazu, A.; Deyashiki, Y.; Nozawa, Y.
    Substrate specificity and kinetic mechanism of Tetrahymena 20alpha-hydroxysteroid dehydrogenase (1995), Adv. Exp. Med. Biol., 372, 249-258.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.149 Bile acids e.g. lithocholic acid, competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena pyriformis
1.1.1.149 Bile acids e.g. lithocholic acid, competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena thermophila
1.1.1.149 cibacron coenzyme analog, competitively with respect to both coenzymes Tetrahymena pyriformis
1.1.1.149 cibacron coenzyme analog, competitively with respect to both coenzymes Tetrahymena thermophila
1.1.1.149 Dienstrol competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena pyriformis
1.1.1.149 Dienstrol competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena thermophila
1.1.1.149 ethyl acetate noncompetitively with respect to NADP+ and (S)-ethyl 3-hydroxybutyrate at backward reaction Tetrahymena pyriformis
1.1.1.149 ethyl acetate noncompetitively with respect to NADP+ and (S)-ethyl 3-hydroxybutyrate at backward reaction Tetrahymena thermophila
1.1.1.149 fatty acids e.g. myristic acid, competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena pyriformis
1.1.1.149 fatty acids e.g. myristic acid, competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena thermophila
1.1.1.149 NADP+ (non)competitively with respect to NADPH and ethyl acetoacetate at forward reaction Tetrahymena pyriformis
1.1.1.149 NADP+ (non)competitively with respect to NADPH and ethyl acetoacetate at forward reaction Tetrahymena thermophila
1.1.1.149 Phenobarbital competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena pyriformis
1.1.1.149 Phenobarbital competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena thermophila
1.1.1.149 quercitrin competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena pyriformis
1.1.1.149 quercitrin competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena thermophila
1.1.1.149 sorbinil competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena pyriformis
1.1.1.149 sorbinil competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena thermophila
1.1.1.149 Tolrestat competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena pyriformis
1.1.1.149 Tolrestat competitive with respect to (S)-ethyl 3-hydroxybutyrate Tetrahymena thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.149 0.0013
-
 n-octyl acetoacetate
-
 Tetrahymena pyriformis
1.1.1.149 0.0015
-
 n-hexyl acetoacetate
-
 Tetrahymena pyriformis
1.1.1.149 0.0016
-
 n-octyl acetoacetate
-
 Tetrahymena thermophila
1.1.1.149 0.002
-
 NADPH +/-0.0001, ethyl acetoacetate Tetrahymena thermophila
1.1.1.149 0.002 0.003 17alpha-hydroxypregnenolone
-
 Tetrahymena pyriformis
1.1.1.149 0.002 0.003 17alpha-hydroxypregnenolone
-
 Tetrahymena thermophila
1.1.1.149 0.0024
-
 n-hexyl acetoacetate
-
 Tetrahymena thermophila
1.1.1.149 0.003
-
 17alpha-hydroxyprogesterone
-
 Tetrahymena pyriformis
1.1.1.149 0.003
-
 17alpha-hydroxyprogesterone
-
 Tetrahymena thermophila
1.1.1.149 0.004
-
 n-butyl acetoacetate
-
 Tetrahymena pyriformis
1.1.1.149 0.004
-
 n-butyl acetoacetate
-
 Tetrahymena thermophila
1.1.1.149 0.005 0.008 2,3-heptanedione
-
 Tetrahymena pyriformis
1.1.1.149 0.005 0.008 2,3-heptanedione
-
 Tetrahymena thermophila
1.1.1.149 0.006
-
 1-phenyl-1,2-propanedione
-
 Tetrahymena pyriformis
1.1.1.149 0.006
-
 1-phenyl-1,2-propanedione
-
 Tetrahymena thermophila
1.1.1.149 0.008
-
 benzyl acetoacetate
-
 Tetrahymena pyriformis
1.1.1.149 0.008
-
 benzyl acetoacetate
-
 Tetrahymena thermophila
1.1.1.149 0.01 0.016 2,3-Hexanedione
-
 Tetrahymena pyriformis
1.1.1.149 0.01 0.016 2,3-Hexanedione
-
 Tetrahymena thermophila
1.1.1.149 0.012
-
 NADP+ +/-0.001, (S)-ethyl 3-hydroxybutyrate Tetrahymena thermophila
1.1.1.149 0.012 0.015 ethyl 2-methylacetoacetate
-
 Tetrahymena pyriformis
1.1.1.149 0.012 0.015 ethyl 2-methylacetoacetate
-
 Tetrahymena thermophila
1.1.1.149 0.013 0.014 17alpha,20alpha-dihydroxyprogesterone
-
 Tetrahymena pyriformis
1.1.1.149 0.013 0.014 17alpha,20alpha-dihydroxyprogesterone
-
 Tetrahymena thermophila
1.1.1.149 0.015 0.018 2,3-Butanedione
-
 Tetrahymena pyriformis
1.1.1.149 0.015 0.018 2,3-Butanedione
-
 Tetrahymena thermophila
1.1.1.149 0.018 0.021 2,3-Pentanedione
-
 Tetrahymena pyriformis
1.1.1.149 0.018 0.021 2,3-Pentanedione
-
 Tetrahymena thermophila
1.1.1.149 0.02 0.032 ethyl acetoacetate
-
 Tetrahymena pyriformis
1.1.1.149 0.02 0.032 ethyl acetoacetate
-
 Tetrahymena thermophila
1.1.1.149 0.02 0.022 acetoacetyl-CoA
-
 Tetrahymena pyriformis
1.1.1.149 0.02 0.022 acetoacetyl-CoA
-
 Tetrahymena thermophila
1.1.1.149 0.025
-
 ethyl pyruvate
-
 Tetrahymena pyriformis
1.1.1.149 0.025
-
 ethyl pyruvate
-
 Tetrahymena thermophila
1.1.1.149 0.027
-
 ethyl 4-chloroacetoacetate
-
 Tetrahymena pyriformis
1.1.1.149 0.027
-
 ethyl 4-chloroacetoacetate
-
 Tetrahymena thermophila
1.1.1.149 0.039 0.045 isatin
-
 Tetrahymena pyriformis
1.1.1.149 0.039 0.045 isatin
-
 Tetrahymena thermophila
1.1.1.149 0.049 0.051 Pyridine-3-aldehyde
-
 Tetrahymena pyriformis
1.1.1.149 0.049 0.051 Pyridine-3-aldehyde
-
 Tetrahymena thermophila
1.1.1.149 0.073
-
 5beta-pregnan-20alpha-ol-3-one
-
 Tetrahymena pyriformis
1.1.1.149 0.092 0.129 1,2-Cyclohexanedione
-
 Tetrahymena thermophila
1.1.1.149 0.117 0.123 methyl acetoacetate
-
 Tetrahymena thermophila
1.1.1.149 0.123
-
 methyl acetoacetate
-
 Tetrahymena pyriformis
1.1.1.149 0.129
-
 1,2-Cyclohexanedione
-
 Tetrahymena pyriformis
1.1.1.149 0.131 0.243 2,4-Pentanedione
-
 Tetrahymena thermophila
1.1.1.149 0.186 0.272 acetoin
-
 Tetrahymena pyriformis
1.1.1.149 0.221 0.254 4-nitrobenzaldehyde
-
 Tetrahymena pyriformis
1.1.1.149 0.221 0.254 4-nitrobenzaldehyde
-
 Tetrahymena thermophila
1.1.1.149 0.222 0.447 ethyl 3-oxovalerate
-
 Tetrahymena thermophila
1.1.1.149 0.23 0.57 3,4-hexanedione
-
 Tetrahymena thermophila
1.1.1.149 0.243
-
 2,4-Pentanedione
-
 Tetrahymena pyriformis
1.1.1.149 0.272
-
 acetoin
-
 Tetrahymena thermophila
1.1.1.149 0.3 0.464 D-glyceraldehyde
-
 Tetrahymena pyriformis
1.1.1.149 0.303 0.654 ethyl benzoylacetate
-
 Tetrahymena thermophila
1.1.1.149 0.356 0.548 methylglyoxal
-
 Tetrahymena pyriformis
1.1.1.149 0.356 0.548 methylglyoxal
-
 Tetrahymena thermophila
1.1.1.149 0.447
-
 ethyl 3-oxovalerate
-
 Tetrahymena pyriformis
1.1.1.149 0.447 0.591 Phenylglyoxal
-
 Tetrahymena thermophila
1.1.1.149 0.464
-
 D-glyceraldehyde
-
 Tetrahymena thermophila
1.1.1.149 0.534 0.587 D-Lactaldehyde
-
 Tetrahymena thermophila
1.1.1.149 0.57
-
 3,4-hexanedione
-
 Tetrahymena pyriformis
1.1.1.149 0.587
-
 D-Lactaldehyde
-
 Tetrahymena pyriformis
1.1.1.149 0.591
-
 Phenylglyoxal
-
 Tetrahymena pyriformis
1.1.1.149 0.654
-
 ethyl benzoylacetate
-
 Tetrahymena pyriformis
1.1.1.149 0.852 1.3 DL-glyceraldehyde 3-phosphate
-
 Tetrahymena thermophila
1.1.1.149 0.945 1.06 Pyridine-4-aldehyde
-
 Tetrahymena thermophila
1.1.1.149 1 1.3 (S)-ethyl 3-hydroxybutyrate
-
 Tetrahymena pyriformis
1.1.1.149 1 1.3 (S)-ethyl 3-hydroxybutyrate
-
 Tetrahymena thermophila
1.1.1.149 1.06
-
 Pyridine-4-aldehyde
-
 Tetrahymena pyriformis
1.1.1.149 1.3
-
 DL-glyceraldehyde 3-phosphate
-
 Tetrahymena pyriformis
1.1.1.149 1.6
-
 trans-benzene-1S,2S-dihydrodiol
-
 Tetrahymena pyriformis
1.1.1.149 1.6
-
 trans-benzene-1S,2S-dihydrodiol
-
 Tetrahymena thermophila
1.1.1.149 2.02 2.9 cis-benzene-1,2-dihydrodiol racemic Tetrahymena pyriformis
1.1.1.149 2.9
-
 cis-benzene-1,2-dihydrodiol racemic Tetrahymena thermophila
1.1.1.149 4.8 5 (S)-methyl 3-hydroxybutyrate
-
 Tetrahymena pyriformis
1.1.1.149 4.8 5 (S)-methyl 3-hydroxybutyrate
-
 Tetrahymena thermophila
1.1.1.149 20
-
 ethyl acetoacetate +/-1 Tetrahymena thermophila
1.1.1.149 34.8 66.3 2,3-Butanediol
-
 Tetrahymena pyriformis
1.1.1.149 50.9 79.7 trans-1S,2S-cyclohexanediol
-
 Tetrahymena pyriformis
1.1.1.149 66.3
-
 2,3-Butanediol
-
 Tetrahymena thermophila
1.1.1.149 79.7
-
 trans-1S,2S-cyclohexanediol
-
 Tetrahymena thermophila
1.1.1.149 1870
-
 (S)-ethyl 3-hydroxybutyrate +/-60 Tetrahymena thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.149 33000
-
 2 * 33000, SDS-PAGE Tetrahymena pyriformis
1.1.1.149 33000
-
 2 * 33000, SDS-PAGE Tetrahymena thermophila
1.1.1.149 68000
-
-
 Tetrahymena pyriformis
1.1.1.149 68000
-
-
 Tetrahymena thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.149 additional information Tetrahymena pyriformis role of enzyme suggested to be for detoxication or metabolism of carbonyl compounds because of broad substrate specificity ?
-
 ?
1.1.1.149 additional information Tetrahymena thermophila role of enzyme suggested to be for detoxication or metabolism of carbonyl compounds because of broad substrate specificity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.149 Tetrahymena pyriformis
-
-
-
1.1.1.149 Tetrahymena thermophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.149 co-purification with aldehyde reductase and dihydrodiol dehydrogenase Tetrahymena pyriformis
1.1.1.149 five steps co-purification with aldehyde reductase and dihydrodiol dehydrogenase, ammonium sulfate precipitation, HPLC Tetrahymena thermophila

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.149 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ aldoketo reductase superfamily Tetrahymena pyriformis
a
1.1.1.149 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ aldoketo reductase superfamily Tetrahymena thermophila
a
1.1.1.149 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ bibi mechanism Tetrahymena pyriformis
a
1.1.1.149 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ bibi mechanism Tetrahymena thermophila
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.149 0.012
-
 +/-0.005 Tetrahymena pyriformis
1.1.1.149 0.024
-
 +/-0.006 Tetrahymena thermophila
1.1.1.149 5
-
-
 Tetrahymena thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.149 (S)-methyl 3-hydroxybutyrate + NADP+
-
 Tetrahymena pyriformis methyl acetoacetate + NADPH + H+
-
 r
1.1.1.149 (S)-methyl 3-hydroxybutyrate + NADP+
-
 Tetrahymena thermophila methyl acetoacetate + NADPH + H+
-
 r
1.1.1.149 1,2-cyclohexanedione + NADP+ 66% activity compared to pyridine 3-aldehyde Tetrahymena pyriformis ?
-
 r
1.1.1.149 1,2-cyclohexanedione + NADP+ 50% activity compared to pyridine 3-aldehyde Tetrahymena thermophila ?
-
 r
1.1.1.149 1-phenyl-1,2-propanedione + NADPH
-
 Tetrahymena pyriformis NADP+ + ?
-
 ?
1.1.1.149 1-phenyl-1,2-propanedione + NADPH
-
 Tetrahymena thermophila NADP+ + ?
-
 ?
1.1.1.149 17alpha-hydroxypregnenolone + NADPH
-
 Tetrahymena pyriformis ? + NADP+
-
 ?
1.1.1.149 17alpha-hydroxypregnenolone + NADPH
-
 Tetrahymena thermophila ? + NADP+
-
 ?
1.1.1.149 17alpha-hydroxyprogesterone + NADPH + H+
-
 Tetrahymena pyriformis 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NADP+
-
 r
1.1.1.149 17alpha-hydroxyprogesterone + NADPH + H+
-
 Tetrahymena thermophila 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NADP+
-
 r
1.1.1.149 2,3-butanediol + NADP+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 2,3-butanediol + NADP+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 2,3-butanedione + NADPH
-
 Tetrahymena pyriformis ? + NADP+
-
 ?
1.1.1.149 2,3-butanedione + NADPH
-
 Tetrahymena thermophila ? + NADP+
-
 ?
1.1.1.149 2,3-heptanedione + NADPH
-
 Tetrahymena pyriformis NADP+ + ?
-
 ?
1.1.1.149 2,3-heptanedione + NADPH
-
 Tetrahymena thermophila NADP+ + ?
-
 ?
1.1.1.149 2,3-hexanedione + NADPH
-
 Tetrahymena pyriformis NADP+ + ?
-
 ?
1.1.1.149 2,3-hexanedione + NADPH
-
 Tetrahymena thermophila NADP+ + ?
-
 ?
1.1.1.149 2,3-pentanedione + NADPH
-
 Tetrahymena pyriformis NADP+ + ?
-
 ?
1.1.1.149 2,3-pentanedione + NADPH
-
 Tetrahymena thermophila NADP+ + ?
-
 ?
1.1.1.149 2,4-pentanedione + NADP+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 2,4-pentanedione + NADP+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 3,4-hexanedione + NADP+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 3,4-hexanedione + NADP+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 4-nitrobenzaldehyde + NADPH
-
 Tetrahymena pyriformis 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.149 4-nitrobenzaldehyde + NADPH
-
 Tetrahymena thermophila 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.149 5beta-pregnan-20alpha-ol-3-one + NADP+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 acetoacetyl-CoA + NADPH
-
 Tetrahymena pyriformis NADP+ + ?
-
 ?
1.1.1.149 acetoacetyl-CoA + NADPH
-
 Tetrahymena thermophila NADP+ + ?
-
 ?
1.1.1.149 acetoin + NADPH + H+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 acetoin + NADPH + H+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 benzyl acetoacetate + NADPH + H+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 benzyl acetoacetate + NADPH + H+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 cis-benzene-1,2-dihydrodiol + NADP+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 cis-benzene-1,2-dihydrodiol + NADP+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 D-glyceraldehyde + NADPH + H+
-
 Tetrahymena pyriformis glycerol + NADP+
-
 r
1.1.1.149 D-glyceraldehyde + NADPH + H+
-
 Tetrahymena thermophila glycerol + NADP+
-
 r
1.1.1.149 D-lactaldehyde + NADPH + H+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 D-lactaldehyde + NADPH + H+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 DL-glyceraldehyde 3-phosphate + NADPH + H+
-
 Tetrahymena pyriformis glycerol 3-phosphate + NADP+
-
 r
1.1.1.149 DL-glyceraldehyde 3-phosphate + NADPH + H+
-
 Tetrahymena thermophila glycerol 3-phosphate + NADP+
-
 r
1.1.1.149 ethyl 2-methylacetoacetate + NADPH
-
 Tetrahymena pyriformis NADP+ + ?
-
 ?
1.1.1.149 ethyl 2-methylacetoacetate + NADPH
-
 Tetrahymena thermophila NADP+ + ?
-
 ?
1.1.1.149 ethyl 3-oxovalerate + NADPH + H+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 ethyl 3-oxovalerate + NADPH + H+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 ethyl 4-chloroacetoacetate + NADPH
-
 Tetrahymena pyriformis NADP+ + ?
-
 ?
1.1.1.149 ethyl 4-chloroacetoacetate + NADPH
-
 Tetrahymena thermophila NADP+ + ?
-
 ?
1.1.1.149 ethyl acetoaetate + NADPH
-
 Tetrahymena thermophila (S)-ethyl 3-hydroxybutyrate + NADP+
-
 r
1.1.1.149 ethyl benzoylacetate + NADPH + H+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 ethyl benzoylacetate + NADPH + H+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 ethyl pyruvate + NADPH
-
 Tetrahymena pyriformis NADP+ + ?
-
 ?
1.1.1.149 ethyl pyruvate + NADPH
-
 Tetrahymena thermophila NADP+ + ?
-
 ?
1.1.1.149 isatin + NADPH
-
 Tetrahymena pyriformis NADP+ + ?
-
 ?
1.1.1.149 isatin + NADPH
-
 Tetrahymena thermophila NADP+ + ?
-
 ?
1.1.1.149 methyl acetoacetate + NADPH + H+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 methyl acetoacetate + NADPH + H+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 methylglyoxal + NADPH
-
 Tetrahymena pyriformis NADP+ + ?
-
 ?
1.1.1.149 methylglyoxal + NADPH
-
 Tetrahymena thermophila NADP+ + ?
-
 ?
1.1.1.149 additional information other substrates: 2,3-butanedione to 2,3-heptanedione, 1-phenyl-1,2-propanedione, ethyl 2-methylacetoacetate, acetoacetyl-CoA, ethyl pyruvate, ethyl 4-chloroacetoacetate, isatin, pyridine-3-aldehyde , not 20beta-hydroxysteroids, 1,2-cyclohexanedione, methyl acetoacetate, 2,4-pentanedione, acetoin, 4-nitrobenzaldehyde, ethyl 3-oxovalerate, 3,4-hexanedione, D-glyceraldehyde, ethyl benzoylacetate, D-lactaldehyde, DL-glceraldehyde 3-phosphate, pyridine-4-aldehyde, (S)-ethyl-3-hydroxybutyrate, trans-benzene-1S,2S-dihydrodiol, cis-benzene-1,2-dihydrodiol, (S)-methyl 3-hydroxybutyrate Tetrahymena pyriformis ?
-
 ?
1.1.1.149 additional information other substrates: 2,3-butanedione to 2,3-heptanedione, 1-phenyl-1,2-propanedione, ethyl 2-methylacetoacetate, acetoacetyl-CoA, ethyl pyruvate, ethyl 4-chloroacetoacetate, isatin, pyridine-3-aldehyde , not 20beta-hydroxysteroids, 1,2-cyclohexanedione, methyl acetoacetate, 2,4-pentanedione, acetoin, 4-nitrobenzaldehyde, ethyl 3-oxovalerate, 3,4-hexanedione, D-glyceraldehyde, ethyl benzoylacetate, D-lactaldehyde, DL-glceraldehyde 3-phosphate, pyridine-4-aldehyde, (S)-ethyl-3-hydroxybutyrate, trans-benzene-1S,2S-dihydrodiol, cis-benzene-1,2-dihydrodiol, (S)-methyl 3-hydroxybutyrate Tetrahymena thermophila ?
-
 ?
1.1.1.149 additional information role of enzyme suggested to be for detoxication or metabolism of carbonyl compounds because of broad substrate specificity Tetrahymena pyriformis ?
-
 ?
1.1.1.149 additional information role of enzyme suggested to be for detoxication or metabolism of carbonyl compounds because of broad substrate specificity Tetrahymena thermophila ?
-
 ?
1.1.1.149 n-butyl acetoacetate + NADPH + H+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 n-butyl acetoacetate + NADPH + H+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 n-hexyl acetoacetate + NADPH + H+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 n-hexyl acetoacetate + NADPH + H+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 n-octyl acetoacetate + NADPH + H+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 n-octyl acetoacetate + NADPH + H+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 phenylglyoxal + NADP+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 phenylglyoxal + NADP+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 pyridine-3-aldehyde + NADPH
-
 Tetrahymena pyriformis NADP+ + ?
-
 ?
1.1.1.149 pyridine-3-aldehyde + NADPH
-
 Tetrahymena thermophila NADP+ + ?
-
 ?
1.1.1.149 pyridine-4-aldehyde + NADP+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 pyridine-4-aldehyde + NADP+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 trans-1S,2S-cyclohexanediol + NADP+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 trans-1S,2S-cyclohexanediol + NADP+
-
 Tetrahymena thermophila ?
-
 r
1.1.1.149 trans-benzene-1S,2S-dihydrodiol + NADP+
-
 Tetrahymena pyriformis ?
-
 r
1.1.1.149 trans-benzene-1S,2S-dihydrodiol + NADP+
-
 Tetrahymena thermophila ?
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.149 dimer 2 * 33000, SDS-PAGE Tetrahymena pyriformis
1.1.1.149 dimer 2 * 33000, SDS-PAGE Tetrahymena thermophila

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.149 NADP+
-
 Tetrahymena pyriformis
1.1.1.149 NADP+
-
 Tetrahymena thermophila
1.1.1.149 NADPH
-
 Tetrahymena pyriformis
1.1.1.149 NADPH
-
 Tetrahymena thermophila