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Literature summary extracted from

  • Zhang, Y.; Dufort, I.; Rheault, P.; Luu-The, V.
    Characterization of a human 20alpha-hydroxysteroid dehydrogenase (2000), J. Mol. Endocrinol., 25, 221-228.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.149 cDNA of skin fibroblast cDNA library inserted into pCMV expression vector, transiently transfected into and expressed in HEK-293 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.149 additional information
-
 additional information rapid activity decrease upon homogenization Homo sapiens
1.1.1.149 0.0006
-
 progesterone NADPH, intact cells Homo sapiens
1.1.1.149 0.0066
-
 progesterone NADPH, cell homogenate Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.149 48150
-
 estimated by 1.3 kb cDNA Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.149 progesterone + NADPH Homo sapiens
-
 20alpha-hydroxyprogesterone + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.149 Homo sapiens
-
-
-
1.1.1.149 Mammalia
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.149 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17alpha-hydroxyprogesterone + NAD(P)H + H+ aldoketo reductase superfamily Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.149 adrenal gland 20alpha-HSD mRNA very low expressed Homo sapiens
-
1.1.1.149 brain 20alpha-HSD mRNA highly expressed Homo sapiens
-
1.1.1.149 gland 20alpha-HSD mRNA highly expressed Mammalia
-
1.1.1.149 HaCaT cell
-
 Homo sapiens
-
1.1.1.149 liver 20alpha-HSD mRNA highly expressed Homo sapiens
-
1.1.1.149 prostate gland 20alpha-HSD mRNA lower expressed Homo sapiens
-
1.1.1.149 skin fibroblast Homo sapiens
-
1.1.1.149 testis 20alpha-HSD mRNA lower expressed Homo sapiens
-
1.1.1.149 uterus 20alpha-HSD mRNA lower expressed Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.149 additional information negligible activity with dehydroepiandrosterone, estrone estradiol, 4-androstenendione, testosterone and diydrotestosterone as substrates Homo sapiens ?
-
 ?
1.1.1.149 progesterone + NAD(P)H NADPH much more efficient, 4-fold lower Km Homo sapiens 20alpha-hydroxyprogesterone + NAD(P)+
-
 ?
1.1.1.149 progesterone + NADPH
-
 Homo sapiens 20alpha-hydroxyprogesterone + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.149 NADH
-
 Homo sapiens
1.1.1.149 NADPH
-
 Homo sapiens