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Literature summary extracted from
- Purification and characterization of 20alpha-hydroxysteroid dehydrogenase from bull testis (1985), J. Steroid Biochem., 23, 1001-1006.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.1.1.149 | - |
Bos taurus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.149 | 0.073 | - |
17-hydroxyprogesterone | - |
Bos taurus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.149 | 34000 | - |
gel filtration | Bos taurus |
| 1.1.1.149 | 40000 | - |
SDS-PAGE | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.149 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.149 | ammonium sulfate precipitation, affinity chromatography, gel filtration | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.149 | testis | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.149 | 0.115 | - |
- |
Bos taurus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.149 | 4°C, in presence of glycerol and DTT | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.149 | 17alpha-hydroxyprogesterone + NAD(P)H + H+ | NADPH preferred | Bos taurus | 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NAD(P)+ | - |
? | |
| 1.1.1.149 | additional information | androstenedione and corticosterone not substrates | Bos taurus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.149 | monomer | 1 * 40000 SDS-PAGE | Bos taurus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.149 | 4 | - |
stable in glycerol and dithiothreitol | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.149 | 5.6 | - |
steroid reduction | Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.149 | NADH | - |
Bos taurus | |
| 1.1.1.149 | NADPH | preferred | Bos taurus | |
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Release 2023.1 (January 2023)
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