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Literature summary extracted from

  • Sato, F.; Takagi, Y.; Shikita, M.
    20alpha-Hydroxysteroid dehydrogenase of porcine testes (1972), J. Biol. Chem., 247, 815-823.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.149 cysteine restoring, also other sulfhydryl compounds, NADH-linked to a greater extent than the NADPH-linked activity Sus scrofa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.149
-
 Sus scrofa

General Stability

EC Number General Stability Organism
1.1.1.149 significant activity loss by repeated thawing and freezing Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.149 Ag+ very powerful Sus scrofa
1.1.1.149 Cu2+ very powerful Sus scrofa
1.1.1.149 Metal ions strong Sus scrofa
1.1.1.149 additional information Ca2+, Fe2+, Mg2+, Mn2+, Cd2+ and Zn2+ no significant effect Sus scrofa
1.1.1.149 p-chloromercuribenzoate also other sufhydryl reagents like 5,5'-dithiobis-2-nitrobenzoic acid and iodoacetamide strong Sus scrofa
1.1.1.149 pyridoxal 5'-phosphate strong, 90% inactivation by 10 mM Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.149 0.022
-
 NADPH 10 mM cysteine, at 4°C until activity decreases to half of the initial value Sus scrofa
1.1.1.149 0.052
-
 NADPH 10 mM cysteine Sus scrofa
1.1.1.149 0.75
-
 NADH 10 mM cysteine Sus scrofa
1.1.1.149 0.75
-
 NADH freshly thawed enzyme Sus scrofa
1.1.1.149 1.1
-
 NADH 10 mM cysteine, at 4°C until activity decreases to half of the initial value Sus scrofa
1.1.1.149 1.1
-
 NADH enzyme solution that has been left standing at 4°C until the activity has decreased to half of the initial value Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.149 35000
-
 gel filtration, sedimentation, sucrose density gradient Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.149 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.149 six step procedure, ammonium sulfate fractionation, chromatography, gel filtration Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.149 testis
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.149 0.081
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.1.1.149 -20°C, porcine testes, 0.1 M phosphate buffer, pH 7.00.1 M phosphate buffer, pH 7.0, several months Sus scrofa
1.1.1.149 4 or -20°C, 2 mg/ml purified protein, 0.1 M phosphate buffer, pH 6-9, several days or for months Sus scrofa
1.1.1.149 4°C or -20°C, ammonium sulfate fraction of purification step 2, over night or several months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.149 17alpha-hydroxy-progesterone + NADH + H+
-
 Sus scrofa 17alpha,20alpha-dihydroxypregn-4-en-3-one + NAD+
-
 r
1.1.1.149 17alpha-hydroxycorticosterone + NADPH less reactive than with 17alpha-hydroxyrogesterone Sus scrofa ? + NADP+
-
 ?
1.1.1.149 17alpha-hydroxyprogesterone + NAD(P)H + H+
-
 Sus scrofa 17alpha,20alpha-dihydroxy-pregn-4-en-3-one + NAD(P)+
-
 r
1.1.1.149 corticosterone + NADPH + H+ less reactive than with 17alpha-hydroxyprogesterone Sus scrofa ? + NADP+
-
 ?
1.1.1.149 additional information no activity toward estrone with NADPH Sus scrofa ?
-
 ?
1.1.1.149 progesterone + NADPH less reactive than with 17alpha-hydroxyprogesterone Sus scrofa 20alpha-hydroxyprogesterone + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.149 monomer
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.149 50
-
 reduction of 17alpha-hydroxyprogesterone Sus scrofa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.149
-
 55 10% of maximal reaction rate at 0°C Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.149 4
-
 at 4°C more stable during purification than at 25°C Sus scrofa
1.1.1.149 25
-
 unstable Sus scrofa
1.1.1.149 55
-
 rapidly inactivated Sus scrofa
1.1.1.149 60
-
 10 min, pH 5.5, complete inactivation of activity of purification step 1 and 2 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.149 5.5 7.5 NADPH Sus scrofa
1.1.1.149 6.8 7.2 NADH Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.149 4 10
-
 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.149 4.2
-
 activity destroyed below Sus scrofa
1.1.1.149 5.5
-
 at pH 5.5 for 30 min, after first purification step Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.149 NADH
-
 Sus scrofa
1.1.1.149 NADPH
-
 Sus scrofa