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Literature summary extracted from

  • Robertson, W.R.; Frost, J.; Hoyer, P.E.; Weinkove, C.
    20alpha-Hydroxysteroid dehydrogenase activity in the rat corpus luteum; a quantitative cytochemical study (1982), J. Steroid Biochem., 17, 237-243.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.149 Dimethyl formamide 5-10% Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.149 17alpha-hydroxyprogesterone most inhibitory at pH 8.0, significant at pH 6.8 Rattus norvegicus
1.1.1.149 20beta-hydroxypregn-4-en-3-one marginally inhibitory at pH 8.0 Rattus norvegicus
1.1.1.149 androstendione most inhibitory at pH 8.0 Rattus norvegicus
1.1.1.149 phenazine methosulfate 0.01-0.3 mg/ml Rattus norvegicus
1.1.1.149 progesterone 1.5 mM marginally inhibitory at pH 8.0, 0.75-1.5 mM reduces activity at pH 6.8 by 46 and 60%, respectively; most inhibitory at pH 8.0, significant at pH 6.8 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.149 0.025
-
 NADP+ 0.01-0.1 mM, 6 min Rattus norvegicus
1.1.1.149 0.3
-
 20alpha-hydroxyprogesterone 0.015-0.1 mM, 6 min Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.149 Rattus norvegicus
-
 adult, female, Sprague-Dawley
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.149 additional information unfixed tissue section, soluble, retention by PVA Rattus norvegicus
-
1.1.1.149 ovary
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.149 additional information no activity with 20beta isomer of 20alpha-hydroxyprogesterone, rising activity with 20alpha-hydroxyprogesterone concentrations over the range 0.05-1.0 mM, increasing activity with NADP+ over the range 0.01-0.2 mM, no NAD+ activity over this concentration range Rattus norvegicus ?
-
 ?
1.1.1.149 progesterone + NADPH
-
 Rattus norvegicus 20alpha-hydroxyprogesterone + NADP+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.149 20
-
 30 min Rattus norvegicus
1.1.1.149 37
-
 6 min Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.149 7.8 8.2
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.149 6.5 9 pH 6.8 favouring progesterone to 20alpha-hydroxyprogesterone reaction and pH 8.0 vice versa Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.149 additional information nitroblue tetrazolium as hydrogen acceptor Rattus norvegicus
1.1.1.149 NADP+
-
 Rattus norvegicus