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Literature summary extracted from

  • Hasnain, S.; Williamson, D.G.
    Properties of the multiple forms of the soluble 17alpha-hydroxy steroid dehydrogenase of rabbit liver (1977), Biochem. J., 161, 279-283.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.148 Epitestosterone substrate inhibition for all isoforms at concentration above 0.003 mM, not observed with other substrates Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.148 0.0002 0.0052 Epitestosterone for six isoforms Oryctolagus cuniculus
1.1.1.148 0.001 0.0051 17alpha-estradiol 3-glucuronide for six isoforms Oryctolagus cuniculus
1.1.1.148 0.0024 0.0088 17alpha-estradiol for six isoforms Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.148 39600
-
 x * 39600, SDS-PAGE, all 8 forms Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.148 Oryctolagus cuniculus
-
 eight isoforms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.148 8 forms, six of them purified to homogenity: IA, IB, IC, IIC, IID, III Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.148 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.148 0.003 0.009 isoform IA, IB, IID, III, 17alpha-estradiol 3-glucuronide Oryctolagus cuniculus
1.1.1.148 0.007 0.015 six isoforms, 17alpha-estradiol Oryctolagus cuniculus
1.1.1.148 0.041 0.2 six isoforms, epitestosterone Oryctolagus cuniculus
1.1.1.148 0.047
-
 isoform IIC, 17alpha-estradiol 3-glucuronide Oryctolagus cuniculus
1.1.1.148 0.18
-
 isoform IC, 17alpha-estradiol 3-glucuronide Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.148 17alpha-estradiol + NAD(P)+
-
 Oryctolagus cuniculus estrone + NAD(P)H
-
 ?
1.1.1.148 17alpha-hydroxy-4-androsten-3-one + NADP+ epitestosterone Oryctolagus cuniculus 4-androsten-3,17-dione + NADPH
-
 r
1.1.1.148 estradiol-17alpha 3-galacturonide + NADP+
-
 Oryctolagus cuniculus estrone 3-galacturonide + NADPH
-
 ?
1.1.1.148 estradiol-17alpha 3-glucoside + NADP+
-
 Oryctolagus cuniculus estrone 3-glucoside + NADPH
-
 ?
1.1.1.148 estradiol-17alpha 3-glucuronide + NADP+
-
 Oryctolagus cuniculus estrone 3-glucuronide + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.148 ? x * 39600, SDS-PAGE, all 8 forms Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.148 NAD+
-
 Oryctolagus cuniculus
1.1.1.148 NADP+
-
 Oryctolagus cuniculus