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Literature summary extracted from

  • Ruis, H.; Hoffmann-Ostendorf, O.
    Enzymic epimerization of sequoyitol to D-pinitol in Trifolium incarnatum (1969), Eur. J. Biochem., 7, 442-448.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.143 DTT 5fold activation Trifolium incarnatum
1.1.1.143 GSH 5fold activation Trifolium incarnatum
1.1.1.143 L-cysteine 5fold activation at 1.3 mM Trifolium incarnatum

General Stability

EC Number General Stability Organism
1.1.1.143 very unstable Trifolium incarnatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.142 Cu2+ 21% inhibition at 0.1 mM Trifolium incarnatum
1.1.1.142 Fe2+ 25% inhibition at 0.1 mM Trifolium incarnatum
1.1.1.142 p-chloromercuribenzoate 25% inhibition at 1 mM Trifolium incarnatum
1.1.1.142 Phenylmercurinitrate 15% inhibition at 0.1 mM Trifolium incarnatum
1.1.1.143 iodoacetate 85% inhibition at 1 mM Trifolium incarnatum
1.1.1.143 p-chloromercuribenzoate complete inhibition at 1 mM Trifolium incarnatum
1.1.1.143 Phenylmercurinitrate complete inhibition at 1 mM Trifolium incarnatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.142 34000
-
 gel filtration Trifolium incarnatum
1.1.1.143 34000
-
 gel filtration Trifolium incarnatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.142 5D-5-O-methyl-chiro-inositol + NADP+ Trifolium incarnatum A-site specificity 5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADPH + H+
-
 r
1.1.1.143 5-O-methyl-myo-inositol + NAD+ Trifolium incarnatum B-specificity 5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.142 Trifolium incarnatum
-
-
-
1.1.1.143 Trifolium incarnatum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.142 partial Trifolium incarnatum
1.1.1.143 partial Trifolium incarnatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.142 plant prior to formation of flowers Trifolium incarnatum
-
1.1.1.143 plant prior to the formation of flowers Trifolium incarnatum
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.142 2°C, 0.01 M phosphate buffer, pH 7.2, little loss of activity within 3 weeks Trifolium incarnatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.142 2,4,6/3,5-pentahydroxycyclohexanone + NADPH
-
 Trifolium incarnatum ? + NADP+
-
 ?
1.1.1.142 5D-5-O-methyl-chiro-inositol + NADP+ A-site specificity Trifolium incarnatum 5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADPH + H+
-
 r
1.1.1.142 D-2,3,5/4,6-pentahydroxycyclohexanone + NADPH
-
 Trifolium incarnatum ? + NADP+
-
 ?
1.1.1.142 dihydroxyacetone + NADPH low activity Trifolium incarnatum propanetriol + NADP+
-
 ?
1.1.1.142 DL-2,3,4,6/5-pentahydroxycyclohexanone + NADPH low activity Trifolium incarnatum ? + NADP+
-
 ?
1.1.1.142 L-2,3,5/4,6-pentahydroxycyclohexanone + NADPH low activity Trifolium incarnatum ? + NADP+
-
 ?
1.1.1.142 L-3-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADPH low activity Trifolium incarnatum ? + NADP+
-
 ?
1.1.1.143 2,4,6/3,5-pentahydroxycyclohexanone + NADH
-
 Trifolium incarnatum ? + NAD+
-
 ?
1.1.1.143 5-O-methyl-myo-inositol + NAD+ B-specificity Trifolium incarnatum 5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADH
-
 r
1.1.1.143 DL-2,3,4,6/5-pentahydroxycyclohexanone + NADH low activity Trifolium incarnatum ? + NAD+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.142 5 9
-
 Trifolium incarnatum
1.1.1.143 5 9
-
 Trifolium incarnatum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.142 NADPH
-
 Trifolium incarnatum
1.1.1.143 NADH
-
 Trifolium incarnatum