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Literature summary extracted from

  • Jabarak, J.
    Isolation and properties of an NAD+-dependent 15-hydroxyprostaglandin dehydrogenase from human placenta (1982), Methods Enzymol., 86, 126-130.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.141 Thionicotinamide adenine dinucleotide 5-33% of the activity with NAD+, depending on substrate Homo sapiens

General Stability

EC Number General Stability Organism
1.1.1.141 glycerol, 50%, stabilizes Homo sapiens
1.1.1.141 NAD+ stabilizes Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.141 arachidonic acid
-
 Homo sapiens
1.1.1.141 Ethacrynic acid
-
 Homo sapiens
1.1.1.141 Prostaglandin B1
-
 Homo sapiens
1.1.1.141 Prostanoic acid
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.141 0.0013
-
 prostaglandin E1
-
 Homo sapiens
1.1.1.141 0.0048
-
 prostaglandin E1
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.141 42000
-
 1 * 42000, SDS-PAGE Homo sapiens
1.1.1.141 51500
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.141 15-hydroxyprostaglandin + NAD+ Homo sapiens physiological inactivation of prostaglandin by catalyzing the first step in catabolism 15-oxoprostaglandin + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.141 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.141
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.141 (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ = (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ single displacement mechanism Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.141 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.141 1.75
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.1.1.141 -20°C, 5 mM potassium phosphate, pH 7.0, 50% glycerol, 1 mM EDTA, 7 months, 10% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.141 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ i.e. prostaglandin E1 Homo sapiens (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?
1.1.1.141 11alpha,15(S)-dihydroxy-9-ketoprost-5,13-dienoic acid + NAD(P)+ specific for NAD+ Homo sapiens 11alpha-hydroxy-9,15-diketoprost-5,13-dienoic acid + NADH
-
 ?
1.1.1.141 15-hydroxyprostaglandin + NAD+ physiological inactivation of prostaglandin by catalyzing the first step in catabolism Homo sapiens 15-oxoprostaglandin + NADH + H+
-
 ?
1.1.1.141 6-ketoprostaglandin F1alpha + NAD+
-
 Homo sapiens 6,15-diketoprostaglandin F1alpha + NADH + H+
-
 ?
1.1.1.141 prostaglandin A1 + NAD+
-
 Homo sapiens 15-ketoprostaglandin A1 + NADH + H+
-
 ?
1.1.1.141 prostaglandin D1 + NAD+ very low activity Homo sapiens 15-ketoprostaglandin D1 + NADH + H+
-
 ?
1.1.1.141 prostaglandin E1 + NAD+
-
 Homo sapiens 15-ketoprostaglandin E1 + NADH + H+
-
 ?
1.1.1.141 prostaglandin F2alpha + NAD+ specific for NAD+ Homo sapiens 15-ketoprostaglandin F2alpha + NADH + H+
-
 ?
1.1.1.141 prostaglandin I2 + NAD+
-
 Homo sapiens 15-ketoprostaglandin I2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.141 monomer 1 * 42000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.141 25
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.141 additional information acetylpyridine dinucleotide can not replace NAD+ Homo sapiens
1.1.1.141 NAD+ specific for NAD+ Homo sapiens
1.1.1.141 NADH
-
 Homo sapiens
1.1.1.141 thio-NAD+ 5-33% of activity with NAD+ depending on substrate Homo sapiens