Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schauder, S.; Schneider, K.H.; Giffhorn, F.
    Polyol metabolism of Rhodobacter sphaeroides: biochemical characterization of a short-chain sorbitol dehydrogenase (1995), Microbiology, 141, 1857-1863.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.14 1 mM dithiothreitol reduces stability Cereibacter sphaeroides
1.1.1.14 30% sucrose stabilizes Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.14 iodoacetate
-
 Cereibacter sphaeroides
1.1.1.14 N-bromosuccinimide
-
 Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.14 0.06
-
 NADH
-
 Cereibacter sphaeroides
1.1.1.14 0.13
-
 NADH
-
 Cereibacter sphaeroides
1.1.1.14 1.5
-
 D-galactitol
-
 Cereibacter sphaeroides
1.1.1.14 6.2
-
 D-glucitol
-
 Cereibacter sphaeroides
1.1.1.14 13
-
 D-tagatose
-
 Cereibacter sphaeroides
1.1.1.14 160
-
 D-fructose
-
 Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.14 29000
-
 2 * 29000, SDS-PAGE Cereibacter sphaeroides
1.1.1.14 61000
-
 Stokes' radius, sedimentation coefficient Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 Cereibacter sphaeroides
-
-
-
1.1.1.14 Cereibacter sphaeroides Si4 / DSM 8371
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.14
-
 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.14 42.3
-
-
 Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
1.1.1.14 4°C, 20 mM potassium phosphate buffer, pH 7.0, 3 months Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-arabitol + NAD+ D-arabinitol is identical, low activity Cereibacter sphaeroides ?
-
 ?
1.1.1.14 D-arabitol + NAD+ D-arabinitol is identical, low activity Cereibacter sphaeroides Si4 / DSM 8371 ?
-
 ?
1.1.1.14 D-fructose + NADH + H+
-
 Cereibacter sphaeroides D-sorbitol + NAD+
-
 ?
1.1.1.14 D-galactitol + NAD+
-
 Cereibacter sphaeroides D-tagatose + NADH
-
 r
1.1.1.14 D-glucitol + NAD+
-
 Cereibacter sphaeroides D-fructose + NADH + H+
-
 r
1.1.1.14 D-mannitol + NAD+ low activity Cereibacter sphaeroides ?
-
 ?
1.1.1.14 D-sorbitol + NAD+
-
 Cereibacter sphaeroides D-fructose + NADH + H+
-
 r
1.1.1.14 D-sorbitol + NAD+
-
 Cereibacter sphaeroides Si4 / DSM 8371 D-fructose + NADH + H+
-
 r
1.1.1.14 D-tagatose + NADH
-
 Cereibacter sphaeroides D-galactitol + NAD+
-
 r
1.1.1.14 D-xylitol + NAD+ low activity Cereibacter sphaeroides ?
-
 ?
1.1.1.14 D-xylulose + NADH low activity Cereibacter sphaeroides ?
-
 ?
1.1.1.14 D-xylulose + NADH low activity Cereibacter sphaeroides Si4 / DSM 8371 ?
-
 ?
1.1.1.14 L-arabitol + NAD+
-
 Cereibacter sphaeroides ?
-
 ?
1.1.1.14 L-arabitol + NAD+
-
 Cereibacter sphaeroides Si4 / DSM 8371 ?
-
 ?
1.1.1.14 L-iditol + NAD+
-
 Cereibacter sphaeroides ?
-
 ?
1.1.1.14 L-iditol + NAD+
-
 Cereibacter sphaeroides Si4 / DSM 8371 ?
-
 ?
1.1.1.14 L-sorbose + NADH low activity Cereibacter sphaeroides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.14 dimer 2 * 29000, SDS-PAGE Cereibacter sphaeroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.14 30
-
 50% loss of activity after 8 days in presence of 30% sucrose Cereibacter sphaeroides
1.1.1.14 40
-
 50% loss of activity after 18 hours in presence of 30% sucrose Cereibacter sphaeroides
1.1.1.14 50
-
 50% loss of activity after 2 min in presence of 30% sucrose Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.14 6 7.2 D-fructose reduction Cereibacter sphaeroides
1.1.1.14 11
-
 sorbitol oxidation Cereibacter sphaeroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.14 6 7.2 less than 50% of maximal activity above and below, sugar reduction Cereibacter sphaeroides
1.1.1.14 9.2 11.6 less than 50% of maximal activity above and below, sorbitol oxidation Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.14 NAD+ specific for Cereibacter sphaeroides
1.1.1.14 NADH specific for Cereibacter sphaeroides