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Literature summary extracted from

  • Bailey, J.P.; Renz, C.; McGuinness, E.T.
    Sorbitol dehydrogenase from horse liver: Purification, characterization and comparative properties (1981), Comp. Biochem. Physiol. B, 69, 909-914.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.14 NAD+ above 7 mM, substrate inhibition Equus caballus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.14 0.007
-
 NADH
-
 Equus caballus
1.1.1.14 0.041
-
 NAD+
-
 Equus caballus
1.1.1.14 0.24
-
 D-sorbitol
-
 Equus caballus
1.1.1.14 330
-
 D-fructose
-
 Equus caballus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.14 26000
-
 x * 26000, SDS-PAGE Ovis aries
1.1.1.14 26000
-
 x * 26000, SDS-PAGE Equus caballus
1.1.1.14 106000
-
 gel filtration Ovis aries
1.1.1.14 110000
-
 gel filtration Equus caballus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 Equus caballus
-
-
-
1.1.1.14 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.14
-
 Equus caballus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.14 liver
-
 Ovis aries
-
1.1.1.14 liver
-
 Equus caballus
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.14 -20°C, stable for 4 months Equus caballus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-fructose + NADH + H+
-
 Ovis aries D-sorbitol + NAD+
-
 ?
1.1.1.14 D-fructose + NADH + H+
-
 Equus caballus D-sorbitol + NAD+
-
 ?
1.1.1.14 D-galactitol + NAD+ low activity Equus caballus D-tagatose + NADH
-
 ?
1.1.1.14 D-mannitol + NAD+
-
 Ovis aries ?
-
 ?
1.1.1.14 D-mannitol + NAD+
-
 Equus caballus ?
-
 ?
1.1.1.14 D-ribitol + NAD+
-
 Ovis aries ?
-
 ?
1.1.1.14 D-ribitol + NAD+
-
 Equus caballus ?
-
 ?
1.1.1.14 D-sorbitol + NAD+
-
 Ovis aries D-fructose + NADH + H+
-
 ?
1.1.1.14 D-sorbitol + NAD+
-
 Equus caballus D-fructose + NADH + H+
-
 ?
1.1.1.14 D-xylitol + NAD+
-
 Ovis aries ?
-
 ?
1.1.1.14 D-xylitol + NAD+
-
 Equus caballus ?
-
 ?
1.1.1.14 L-arabitol + NAD+
-
 Ovis aries ?
-
 ?
1.1.1.14 L-arabitol + NAD+ low activity Equus caballus ?
-
 ?
1.1.1.14 L-iditol + NAD+
-
 Ovis aries ?
-
 ?
1.1.1.14 L-iditol + NAD+
-
 Equus caballus ?
-
 ?
1.1.1.14 L-sorbose + NADH
-
 Equus caballus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.14 ? x * 26000, SDS-PAGE Ovis aries
1.1.1.14 ? x * 26000, SDS-PAGE Equus caballus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.14 20
-
 30% loss of activity after 3 days, 70% loss of activity after 6 days, 100% loss of activity after 10 days Equus caballus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.14 8.8
-
 D-fructose reduction Equus caballus
1.1.1.14 9.6
-
 sorbitol oxidation Equus caballus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.14 NAD+
-
 Ovis aries
1.1.1.14 NAD+ specific for Equus caballus
1.1.1.14 NADH
-
 Ovis aries
1.1.1.14 NADH specific for Equus caballus