Literature summary extracted from

Horer, S.; Stoop, J.; Mooibroek, H.; Baumann, U.; Sassoon, J.
The crystallographic structure of the mannitol 2-dehydrogenase NADP+ binary complex from Agaricus bisporus (2001), J. Biol. Chem., 276, 27555-27561.

Application

EC Number	Application	Comment	Organism
1.1.1.138	synthesis	commercial mannitol production as alternatives to less efficient chemical reduction of fructose	Agaricus bisporus

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.138	expression in Escherichia coli	Agaricus bisporus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.1.1.138	binary complex with NADP+	Agaricus bisporus
1.1.1.138	binary complex with NADP+ at 1.5 A resolution, catalytic triad is assigned to Ser149, Tyr169 and Lys173	Agaricus bisporus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.138	D-fructose + NADPH	Agaricus bisporus	-	D-mannitol + NADP+	-	r
1.1.1.138	D-mannitol + NADP+	Agaricus bisporus	mannitol serves as storage carbon, plays a critical role in growth, fruit body development, osmoregulation and salt tolerance	D-fructose + NADPH + H+	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.138	Agaricus bisporus	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.1.1.138	fruit body	-	Agaricus bisporus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.138	D-fructose + NADPH	-	Agaricus bisporus	D-mannitol + NADP+	-	r
1.1.1.138	D-mannitol + NADP+	mannitol serves as storage carbon, plays a critical role in growth, fruit body development, osmoregulation and salt tolerance	Agaricus bisporus	D-fructose + NADPH + H+	-	r

Subunits

EC Number	Subunits	Comment	Organism
1.1.1.138	tetramer	data from X-ray structure	Agaricus bisporus
1.1.1.138	tetramer	crystallization data, residues Ser149, Tyr169, Lys173 form a catalytic triad	Agaricus bisporus

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.138	MtDH	-	Agaricus bisporus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.138	NADP+	specific for NADP+	Agaricus bisporus
1.1.1.138	NADPH	-	Agaricus bisporus

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Release 2023.1 (January 2023)