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Literature summary extracted from

  • Morton, N.; Dickerson, A.G.; Hammond, J.B.W.
    Mannitol metabolism in Agaricus bisporus: Purification and properties of mannitol dehydrogenase (1985), J. Gen. Microbiol., 131, 2885-2890.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.138 NADP+ D-fructose reduction, NADP+/NADPH ratio of 38 necessary for 50% inhibition Agaricus bisporus
1.1.1.138 NADPH D-mannitol oxidation, NADPH/NADP+ ratio of 0.1 necessary for 50% inhibition Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.138 0.036
-
 NADP+ pH 7.0 Agaricus bisporus
1.1.1.138 0.0385
-
 NADPH pH 7.0 Agaricus bisporus
1.1.1.138 0.082
-
 NADP+ pH 10.25 Agaricus bisporus
1.1.1.138 16.2
-
 D-mannitol pH 10.25 and pH 7.0 Agaricus bisporus
1.1.1.138 1200
-
 D-fructose at pH 7.0 Agaricus bisporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.138 29500
-
 4 * 29500, SDS-PAGE Agaricus bisporus
1.1.1.138 115000 130000 gel filtration, rate zonal ultracentrifugation Agaricus bisporus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.138 D-fructose + NADPH Agaricus bisporus
-
 D-mannitol + NADP+
-
 r
1.1.1.138 D-mannitol + NADP+ Agaricus bisporus
-
 D-fructose + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.138 Agaricus bisporus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.138 to homogeneity, chromatography techniques Agaricus bisporus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.138 fruit body
-
 Agaricus bisporus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.138 194.4
-
-
 Agaricus bisporus

Storage Stability

EC Number Storage Stability Organism
1.1.1.138 -20°C, 10 mM Tris-HCl, pH 7.5, 10 mM 2-mercaptoethanol, 0.05 mM PMSF, 50%, v/v, glycerol, 1 month Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.138 D-fructose + NADPH
-
 Agaricus bisporus D-mannitol + NADP+
-
 r
1.1.1.138 D-mannitol + NADP+
-
 Agaricus bisporus D-fructose + NADPH + H+
-
 r
1.1.1.138 L-sorbitol + NADP+ 7.7% of the activity compared to D-mannitol Agaricus bisporus L-sorbose + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.138 tetramer 4 * 29500, SDS-PAGE Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.138 7
-
 D-fructose reduction Agaricus bisporus
1.1.1.138 10.25
-
 D-mannitol oxidation Agaricus bisporus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.138 NADP+ cannot be replaced by NAD+ Agaricus bisporus
1.1.1.138 NADPH cannot be replaced by NADH Agaricus bisporus