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Literature summary extracted from

  • Adachi, O.; Toyama, H.; Matsushita, K.
    Crystalline NADP-dependent D-mannitol dehydrogenase from Gluconobacter suboxydans (1999), Biosci. Biotechnol. Biochem., 63, 402-407.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.138 analysis determination of D-fructose in the presence of other sugars Gluconobacter oxydans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.138 ammonium sulfate precipitation Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.138 0.019
-
 NADPH
-
 Gluconobacter oxydans
1.1.1.138 0.025
-
 NADP+
-
 Gluconobacter oxydans
1.1.1.138 10
-
 D-mannitol
-
 Gluconobacter oxydans
1.1.1.138 12
-
 D-fructose
-
 Gluconobacter oxydans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.138 50000
-
 gel filtration Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.138 D-fructose + NADPH Gluconobacter oxydans
-
 D-mannitol + NADP+
-
 ?
1.1.1.138 D-fructose + NADPH Gluconobacter oxydans IFO 12528
-
 D-mannitol + NADP+
-
 ?
1.1.1.138 D-mannitol + NADP+ Gluconobacter oxydans
-
 D-fructose + NADPH + H+
-
 r
1.1.1.138 D-mannitol + NADP+ Gluconobacter oxydans IFO 12528
-
 D-fructose + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.138 Gluconobacter oxydans
-
-
-
1.1.1.138 Gluconobacter oxydans IFO 12528
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.138 to crystalline state, chromatography techniques, crystallization Gluconobacter oxydans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.138 cell culture
-
 Gluconobacter oxydans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.138 235
-
-
 Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.138 D-fructose + NADPH
-
 Gluconobacter oxydans D-mannitol + NADP+
-
 ?
1.1.1.138 D-fructose + NADPH
-
 Gluconobacter oxydans IFO 12528 D-mannitol + NADP+
-
 ?
1.1.1.138 D-mannitol + NADP+
-
 Gluconobacter oxydans D-fructose + NADPH + H+
-
 r
1.1.1.138 D-mannitol + NADP+
-
 Gluconobacter oxydans IFO 12528 D-fructose + NADPH + H+
-
 r
1.1.1.138 L-sorbitol + NADP+ 4% of the activity compared to D-mannitol Gluconobacter oxydans L-sorbose + NADPH
-
 r
1.1.1.138 L-sorbitol + NADP+ 4% of the activity compared to D-mannitol Gluconobacter oxydans IFO 12528 L-sorbose + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.138 monomer 1 * 50000, SDS-PAGE Gluconobacter oxydans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.138 D-mannitol dehydrogenase, NADP-dependent
-
 Gluconobacter oxydans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.138 50
-
 stable over 30 min Gluconobacter oxydans
1.1.1.138 53
-
 20-50% retaining activity after 20 min Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.138 6 8 reduction of D-fructose Gluconobacter oxydans
1.1.1.138 9 10 oxidation of D-mannitol Gluconobacter oxydans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.138 3.5 9.5 stable within Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.138 NADP+ specific for NADP+ Gluconobacter oxydans
1.1.1.138 NADPH
-
 Gluconobacter oxydans