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Literature summary extracted from

  • Graninger, M.; Nidetzky, B.; Heinrichs, D.E.; Whitfield, C.; Messner, P.
    Characterization of dTDP-4-dehydrorhamnose 3,5-epimerase and dTDP-4-dehydrorhamnose reductase, required for dTDP-L-rhamnose biosynthesis in Salmonella enterica serovar typhimurium LT2 (1999), J. Biol. Chem., 274, 25069-25077.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.133 overexpressed in Escherichia coli BL21(lambdaDE3) Salmonella enterica
5.1.3.13 expression in Escherichia coli Salmonella enterica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.133 EDTA up to 0.3 mM, 70% loss of enzyme activity Salmonella enterica
1.1.1.133 additional information full activity restored with up to 0.3 mM MgCl2 Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.133 0.106
-
 NADH +/-0.018, pH 7.0 Salmonella enterica
1.1.1.133 0.21
-
 NADH +/-0.004, pH 7.0 Salmonella enterica
5.1.3.13 0.71
-
 dTDP-6-deoxy-D-xylo-4-hexulose pH 7.0, 25°C Salmonella enterica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.133 32600
-
 calculated from nucleotide sequence data Salmonella enterica
1.1.1.133 34800
-
 SDS-PAGE Salmonella enterica
1.1.1.133 41500
-
 gel filtration Salmonella enterica
5.1.3.13 40600
-
 gel filtration Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.13 additional information Salmonella enterica the enzyme is required for dTDP-L-rhamnose biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.133 Salmonella enterica
-
 serovar typhimurium LT2
-
5.1.3.13 Salmonella enterica
-
 serovar typhimurium LT2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.133 affinity and ion exchange chromatography Salmonella enterica
5.1.3.13
-
 Salmonella enterica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.133 44
-
-
 Salmonella enterica

Storage Stability

EC Number Storage Stability Organism
1.1.1.133 -20°C, 44% glycerol Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.133 dTDP-4-dehydro-6-deoxy-D-glucose + NADH + H+
-
 Salmonella enterica dTDP-beta-L-rhamnose + NAD+
-
 ?
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose + NAD(P)H
-
 Salmonella enterica dTDP-L-rhamnose + NAD(P)+
-
 r
5.1.3.13 dTDP-6-deoxy-D-xylo-4-hexulose
-
 Salmonella enterica dTDP-6-deoxy-L-lyxo-4-hexulose
-
 ?
5.1.3.13 additional information the enzyme is required for dTDP-L-rhamnose biosynthesis Salmonella enterica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.13 dimer
-
 Salmonella enterica

Synonyms

EC Number Synonyms Comment Organism
1.1.1.133 dTDP-4-dehydrorhamnose reductase
-
 Salmonella enterica
1.1.1.133 thymidine diphosphate-4-dehydrorhamnose reductase
-
 Salmonella enterica
5.1.3.13 RmlC
-
 Salmonella enterica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.133 30
-
 stable below Salmonella enterica
1.1.1.133 37
-
 pH 7.0, 75% activity loss Salmonella enterica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.133 25
-
 NADH +/-48 Salmonella enterica
1.1.1.133 29
-
 NADPH +/-66 Salmonella enterica
5.1.3.13 39
-
 dTDP-6-deoxy-D-xylo-4-hexulose pH 7.0, 25°C Salmonella enterica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.133 6.5
-
 reduction of dTDP-6-deoxy-L-lyxo-4-hexulose Salmonella enterica
1.1.1.133 10
-
 oxidation of dTDP-L-rhamnose Salmonella enterica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.133 7
-
 37°C, 20 min, 75% activity loss Salmonella enterica

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.133 NAD+
-
 Salmonella enterica
1.1.1.133 NADH preferred Salmonella enterica
1.1.1.133 NADP+
-
 Salmonella enterica
1.1.1.133 NADPH
-
 Salmonella enterica