Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tatnell, P.J.; Russell, N.J.; Gacesa, P.
    Overexpression of the GDP-mannose dehydrogenase gene from Pseudomonas aeruginosa (1996), Biochem. Soc. Trans., 24, 405S.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.132 Pseudomonas aeruginosa
-
 enzyme overproduced using 3 different plasmid vectors containing algD under control of the tac promoter expressed in non-mucoid strain PAO1 and mucoid strain PAO568, pPT3 and pPT4 are forward and reverse clones contain leader sequence with possible transcriptional/translational regulatory sequences of algD gene, no significant activity with pMMB66EH and pPT4 in either IPTG induced or non induced Escherichia coli, mucoid and non-mucoid Pseudomonas aeruginosa strains
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.132 0.00015
-
 +/-0.00001, S17-1/pPT4, with IPTG Pseudomonas aeruginosa
1.1.1.132 0.00017
-
 +/-0.00002, S17-1/pMMB66EH and S17-1/pPT4 without IPTG Pseudomonas aeruginosa
1.1.1.132 0.00022
-
 +/-0.00001, S17-1/pMMB66EH, with IPTG Pseudomonas aeruginosa
1.1.1.132 0.00034
-
 +/-0.00006, S17-1/pPT3, without IPTG Pseudomonas aeruginosa
1.1.1.132 0.0009
-
 +/-0.0003, PAO1/pMMB66EH, without IPTG Pseudomonas aeruginosa
1.1.1.132 0.0009
-
 +/-0.0002, PAO1/pMMB66EH, with IPTG Pseudomonas aeruginosa
1.1.1.132 0.013
-
 +/-0.003, PAO1/pPT3, without IPTG Pseudomonas aeruginosa
1.1.1.132 0.014
-
 +/-0.0014, PAO1/pPT4, with IPTG Pseudomonas aeruginosa
1.1.1.132 0.0147
-
 +/-0.0014, PAO1/pPT4, without IPTG Pseudomonas aeruginosa
1.1.1.132 0.0176
-
 +/-0.0009, PAO568/pMMB66EH, with IPTG Pseudomonas aeruginosa
1.1.1.132 0.0182
-
 +/-0.0003, PAO568/pMMB66EH, without IPTG Pseudomonas aeruginosa
1.1.1.132 0.0223
-
 +/-0.0011, PAO568/pPT3, without IPTG Pseudomonas aeruginosa
1.1.1.132 0.0251
-
 +/-0.0027, PAO568/pPT4, without IPTG Pseudomonas aeruginosa
1.1.1.132 0.0254
-
 +/-0.004, PAO568/pPT4, with IPTG Pseudomonas aeruginosa
1.1.1.132 0.1895
-
 +/-0.00702, S17-1/pPT3, with IPTG Pseudomonas aeruginosa
1.1.1.132 0.2041
-
 +/-0.00932, PAO1/pPT3, with IPTG Pseudomonas aeruginosa
1.1.1.132 0.2378
-
 +/-0.0552, PAO568/pPT3, with IPTG Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.132 GDP-mannose + NAD+ + H2O
-
 Pseudomonas aeruginosa GDP-mannuronate + NADH
-
 ir

Subunits

EC Number Subunits Comment Organism
1.1.1.132 hexamer
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.132 NAD+
-
 Pseudomonas aeruginosa