Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Martins, L.O.; Sa-Correia, I.
    Alginate biosynthesis in mucoid recombinants of Pseudomonas aeruginosa overproducing GDP-mannose dehydrogenase (1991), Enzyme Microb. Technol., 13, 385-389.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.132 Pseudomonas aeruginosa
-
 mucoid strains of cystic fibrosis patients 8821 and its high-alginate-producing spontaneously variant 8821M, enzyme overproduced using 2 different plasmid vectors containing algD under control of the tac promoter mobilized into both strains
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.132 additional information
-
 increase of activity has only limited effect on the stimulation of alginate synthesis, existance of other rate-limiting enzymatic step suggested Pseudomonas aeruginosa
1.1.1.132 0.0058
-
 +/-0.002, 8821/pMMB24, not IPTG induced Pseudomonas aeruginosa
1.1.1.132 0.0128
-
 +/-0.0018, 8821/pVD211, not IPTG induced Pseudomonas aeruginosa
1.1.1.132 0.0226
-
 +/-0.0025, 8821/pVD211, induced by 0.5 mM IPTG Pseudomonas aeruginosa
1.1.1.132 0.0265
-
 +/-0.003, 8821/pVD211, induced by 3 mM IPTG Pseudomonas aeruginosa
1.1.1.132 0.028
-
 +/-0.0029, 8821M/pMMB24, not IPTG induced Pseudomonas aeruginosa
1.1.1.132 0.0579
-
 +/-0.0029, 8821/pVD211, not IPTG induced Pseudomonas aeruginosa
1.1.1.132 0.084
-
 +/-0.0019, 8821/pVD211, induced by 0.5 mM IPTG Pseudomonas aeruginosa
1.1.1.132 0.0942
-
 +/-0.004, 8821/pVD211, induced by 3 mM IPTG Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.132 GDP-mannose + NAD+ + H2O
-
 Pseudomonas aeruginosa GDP-mannuronate + NADH
-
 ir

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.132 NAD+
-
 Pseudomonas aeruginosa