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Literature summary extracted from
- GDP-mannose dehydrogenase from Arthrobacter (1966), Methods Enzymol., 8, 285-287.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.132 | 0.017 | - |
GDP-mannose | - |
Arthrobacter sp. |  |
| 1.1.1.132 | 0.084 | - |
deoxy-GDP-D-mannose | - |
Arthrobacter sp. | |
| 1.1.1.132 | 0.26 | - |
NAD+ | cosubstrate 0.3 mM GDP-mannose | Arthrobacter sp. | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.132 | Arthrobacter sp. | - |
NRRL B1973 | - |
| 1.1.1.132 | Arthrobacter sp. NRRL B1973 | - |
NRRL B1973 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.132 | protamine sulfate fractionation | Arthrobacter sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.132 | 0.39 | - |
- |
Arthrobacter sp. |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.132 | -15°C, protein concentration above 1 mg/ml, at least 1 month | Arthrobacter sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.132 | Deoxy-GDP-D-mannose + 2 NAD+ + H2O | - |
Arthrobacter sp. | deoxy-GDP-D-mannuronate + 2 NADH + 2 H+ | - |
r | |
| 1.1.1.132 | Deoxy-GDP-D-mannose + 2 NAD+ + H2O | - |
Arthrobacter sp. NRRL B1973 | deoxy-GDP-D-mannuronate + 2 NADH + 2 H+ | - |
r | |
| 1.1.1.132 | deoxy-GDP-D-mannose + NAD+ + H2O | - |
Arthrobacter sp. | deoxy-GDP-D-mannuronate + NADH | - |
? | |
| 1.1.1.132 | deoxy-GDP-D-mannose + NAD+ + H2O | - |
Arthrobacter sp. NRRL B1973 | deoxy-GDP-D-mannuronate + NADH | - |
? | |
| 1.1.1.132 | GDP-mannose + NAD+ + H2O | - |
Arthrobacter sp. | GDP-mannuronate + NADH | - |
? | |
| 1.1.1.132 | GDP-mannose + NAD+ + H2O | - |
Arthrobacter sp. NRRL B1973 | GDP-mannuronate + NADH | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.132 | 8.2 | - |
Tris buffer | Arthrobacter sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.132 | NAD+ | - |
Arthrobacter sp. | |
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Release 2023.1 (January 2023)
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