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Literature summary extracted from

  • Volk, W.A.; Larsen, J.L.
    beta-Keto-L-gulonic acid as an intermediate in the bacterial metabolism of ascorbic acid (1962), J. Biol. Chem., 237, 2454-2457.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.130 p-chloromercuribenzoate 32% inhibition at 1 mM, not reversal by GSH Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.130 0.016
-
 NADH
-
 Klebsiella aerogenes
1.1.1.130 0.022
-
 NADPH
-
 Klebsiella aerogenes
1.1.1.130 1.16
-
 (4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate
-
 Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.130 (4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H Klebsiella aerogenes
-
 3-dehydro-L-gulonate + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.130 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.130 partial Klebsiella aerogenes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.130 cell culture
-
 Klebsiella aerogenes
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.130 8.06
-
-
 Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
1.1.1.130 0°C, 0.03 M Tris-HCl, pH 7.7, 0.62 mg protein/ml, 85% retaining activity in 4 weeks Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.130 (4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H
-
 Klebsiella aerogenes 3-dehydro-L-gulonate + NAD(P)+
-
 ?
1.1.1.130 2,3-diketogulonic acid + NADH + H+
-
 Klebsiella aerogenes 3-dehydro-L-gulonate + NAD+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.130 6.5
-
-
 Klebsiella aerogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.130 5.2 8.4 50% of maximal activity at pH 5.2 and pH 8.4 Klebsiella aerogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.130 NADH twice effective compared to NADPH Klebsiella aerogenes
1.1.1.130 NADPH NADH 2fold more effective Klebsiella aerogenes