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Literature summary extracted from

  • Wolfe, G.R.; Smith, C.A.; Hendrix, D.L.; Salvucci, M.E.
    Molecular basis for thermoprotection in Bemisia: structural differences between whitefly ketose reductase and other medium-chain dehydrogenases/reductases (1999), Insect Biochem. Mol. Biol., 29, 113-120.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.123 expressed in Escherichia coli XL1-Blue Bemisia argentifolii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.123 additional information addition of Arg and loss of Asp decrease the electric potential of the adenosine ribose-binding-pocket, creating an environment favorable for NADPH-binding, the replacement of an Asp with an Ala and the adjacent Leu with an Arg change the potential Bemisia argentifolii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.123 Zn2+ catalytical and structural zinc-coordinating residues Bemisia argentifolii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.123 additional information
-
 amino acid sequence has 60% identity with sheep liver, 41% with Bombyx mori sorbitol dehydrogenase and 21% with horse liver alcohol dehydrogenase Bemisia argentifolii
1.1.1.123 38160
-
 calculation from polypeptide sequence of 352 amino acids Bemisia argentifolii
1.1.1.123 38700
-
 4 * 38700, SDS-PAGE Bemisia argentifolii
1.1.1.123 39000
-
 4 * 39000, recombinant enzyme, SDS-PAGE Bemisia argentifolii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.123 Bemisia argentifolii
-
 adult silverleaf whitefly
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.123
-
 Bemisia argentifolii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.123 fructose + NADPH
-
 Bemisia argentifolii sorbitol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.123 tetramer 4 * 38700, SDS-PAGE Bemisia argentifolii
1.1.1.123 tetramer 4 * 39000, recombinant enzyme, SDS-PAGE Bemisia argentifolii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.123 25 41 sorbitol content increases in a temperature dependent manner Bemisia argentifolii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.123 NADPH almost exclusive preference/requirement for NADPH Bemisia argentifolii