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Literature summary extracted from

  • Yagi, S.; Kobayahi, K.; Sonoyama, T.
    Purification and properties of 5-keto-D-fructose reductase from a mutant strain derived from Corynebacterium sp. (1989), J. Ferment. Bioeng., 67, 212-214.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.123 5.9
-
5-keto-D-fructose pH 7.0, 30°C Corynebacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.123 33000
-
gel filtration Corynebacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.123 Corynebacterium sp.
-
mutant strain, SHS 752001
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.123 ammonium sulfate precipitation, DEAE-Sepharose-, Gel Red A-, Phenyl Sepharose column chromatography Corynebacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.123 67.3
-
-
Corynebacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.123 5-keto-D-fructose + NADPH
-
Corynebacterium sp. L-sorbose + NADP+
-
ir

Subunits

EC Number Subunits Comment Organism
1.1.1.123 monomer 1 * 33000, SDS-PAGE Corynebacterium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.123
-
38 activity decreases gradually with increasing temperature from 0°C to 27°C, falls rapidly above 27°C and is completely lost at 38°C Corynebacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.123 6 7
-
Corynebacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.123 5 9 pH 5: about 20% of activity maximum, pH 9: about 35% of activity maximum Corynebacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.123 additional information no activity with NADH Corynebacterium sp.
1.1.1.123 NADPH
-
Corynebacterium sp.