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Literature summary extracted from

  • Endo, M.; Hiyama, N.
    Isolation and characterization of L-fucose dehydrogenase from rabbit liver (1979), J. Biochem., 86, 1559-1565.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.122 Cd2+ inhibitory Oryctolagus cuniculus
1.1.1.122 Hg2+
-
 Oryctolagus cuniculus
1.1.1.122 iodoacetic acid 10% inhibition at 1 mM Oryctolagus cuniculus
1.1.1.122 p-chloromercuribenzoate 48% inhibition at 1 mM Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.122 0.07
-
 NAD+
-
 Oryctolagus cuniculus
1.1.1.122 0.15
-
 6-deoxy-L-galactose
-
 Oryctolagus cuniculus
1.1.1.122 1.4
-
 D-arabinose
-
 Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.122 92000
-
 gel filtration Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.122 6-deoxy-L-galactose + NAD+ Oryctolagus cuniculus irreversible because of spontaneous conversion to L-fuconic acid during incubation 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ir
1.1.1.122 6-deoxy-L-galactose + NAD+ Oryctolagus cuniculus i.e. L-fucose 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ir
1.1.1.122 D-arabinose + NAD+ Oryctolagus cuniculus 84% of the activity compared to L-fucose D-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.122 D-xylose + NAD+ Oryctolagus cuniculus 2.1% of the activity compared to L-fucose D-xylono-1,5-lactone + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.122 Oryctolagus cuniculus
-
 rabbit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.122 to homogeneity, chromatography steps, preparative gel electrophoresis, affinity chromatography Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.122 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.122 1.03
-
 purified enzyme Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
1.1.1.122 -20°C, presence of NAD+, 1 year Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.122 6-deoxy-L-galactose + NAD+ irreversible because of spontaneous conversion to L-fuconic acid during incubation Oryctolagus cuniculus 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ir
1.1.1.122 6-deoxy-L-galactose + NAD+ i.e. L-fucose Oryctolagus cuniculus 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ir
1.1.1.122 D-arabinose + NAD+ 84% of the activity compared to L-fucose Oryctolagus cuniculus D-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.122 D-xylose + NAD+ 2.1% of the activity compared to L-fucose Oryctolagus cuniculus D-xylono-1,5-lactone + NADH + H+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.122 50
-
-
 Oryctolagus cuniculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.122 50
-
 half-life: 30 min Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.122 10
-
-
 Oryctolagus cuniculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.122 5 12 stable between Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.122 NAD+ NAD-specific Oryctolagus cuniculus