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Literature summary extracted from
- L-Fucose dehydrogenase from sheep liver (1975), Methods Enzymol., 41B, 173-177.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.122 | stable to refrigeration at 5°C for a week | Ovis aries aries |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.122 | 0.01 | - |
NAD+ | at pH 10.4 | Ovis aries aries |  |
| 1.1.1.122 | 0.04 | - |
NAD+ | at pH 8.6 | Ovis aries aries | |
| 1.1.1.122 | 0.07 | - |
6-deoxy-L-galactose | at pH 8.6 | Ovis aries aries | |
| 1.1.1.122 | 0.4 | - |
D-arabinose | at pH 8.6 | Ovis aries aries | |
| 1.1.1.122 | 1.5 | - |
6-deoxy-L-galactose | at pH 10.4 | Ovis aries aries | |
| 1.1.1.122 | 7.3 | - |
D-arabinose | at pH 10.4 | Ovis aries aries | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.122 | 96000 | - |
electro focusing | Ovis aries aries |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.122 | 2-deoxy-D-ribose + NAD+ | Ovis aries aries | 10% of the activity compared to L-fucose or D-arabinose | ? + NADH | - |
? | |
| 1.1.1.122 | 6-deoxy-L-galactose + NAD+ | Ovis aries aries | ordered bi-bi-reaction mechanism | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
? | |
| 1.1.1.122 | 6-deoxy-L-galactose + NAD+ | Ovis aries aries | i.e. L-fucose | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
? | |
| 1.1.1.122 | D-arabinose + NAD+ | Ovis aries aries | - |
D-arabinono-1,4-lactone + NADH | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.122 | Ovis aries aries | - |
sheep | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.122 | to homogeneity, chromatography techniques | Ovis aries aries |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.122 | liver | - |
Ovis aries aries | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.122 | -20°C, minimum concentration: 1 mg/ml protein, 1 month | Ovis aries aries |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.122 | 2-deoxy-D-ribose + NAD+ | 10% of the activity compared to L-fucose or D-arabinose | Ovis aries aries | ? + NADH | - |
? | |
| 1.1.1.122 | 6-deoxy-L-galactose + NAD+ | ordered bi-bi-reaction mechanism | Ovis aries aries | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
? | |
| 1.1.1.122 | 6-deoxy-L-galactose + NAD+ | i.e. L-fucose | Ovis aries aries | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
? | |
| 1.1.1.122 | D-arabinose + NAD+ | - |
Ovis aries aries | D-arabinono-1,4-lactone + NADH | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.122 | 60 | - |
stable up to | Ovis aries aries |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.122 | 10.4 | - |
ionic strength: 0.05 | Ovis aries aries |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.122 | NAD+ | - |
Ovis aries aries | |
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Release 2023.1 (January 2023)
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