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Literature summary extracted from

  • Dahms, A.S.; Anderson, R.L.
    D-Fucose metabolism in a pseudomonad. I. Oxidation of D-fucose to D-fucono-delta-lactone by a D-aldohexose dehydrogenase (1972), J. Biol. Chem., 247, 2222-2227.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.121 Ca2+
-
 Pseudomonas sp.
1.1.1.121 Co2+
-
 Pseudomonas sp.
1.1.1.121 Cu2+
-
 Pseudomonas sp.
1.1.1.121 Fe2+
-
 Pseudomonas sp.
1.1.1.121 additional information no inhibition by 6.7 mM EDTA or 4.0 mM NADP+ Pseudomonas sp.
1.1.1.121 NH4+
-
 Pseudomonas sp.
1.1.1.121 Ni2+
-
 Pseudomonas sp.
1.1.1.121 Zn2+
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.121 0.08
-
 NAD+
-
 Pseudomonas sp.
1.1.1.121 0.86
-
 D-glucose
-
 Pseudomonas sp.
1.1.1.121 1.6
-
 D-galactose
-
 Pseudomonas sp.
1.1.1.121 1.6
-
 2-deoxy-D-glucose
-
 Pseudomonas sp.
1.1.1.121 2.4
-
 D-altrose
-
 Pseudomonas sp.
1.1.1.121 4.5
-
 D-mannose
-
 Pseudomonas sp.
1.1.1.121 5.8
-
 D-fucose
-
 Pseudomonas sp.
1.1.1.121 6.3
-
 2-deoxy-D-galactose
-
 Pseudomonas sp.
1.1.1.121 13
-
 D-allose
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.121 D-aldose + NAD+ Pseudomonas sp. broad substrate specificity D-aldonolactone + NADH
-
 ?
1.1.1.121 D-aldose + NAD+ Pseudomonas sp. D-aldoses of pyranose ring size D-aldonolactone + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.121 Pseudomonas sp.
-
 MSU-1
-
1.1.1.121 Pseudomonas sp. MSU-1
-
 MSU-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.121 MSU-1 Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.121 cell culture cells grown on various sugars, D-fucose and D-glucose are effective inducers of D-aldohexose dehydrogenase, D-galactose induces only slightly, L-arabinose is no inducer Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.121 13.8
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.121 -20°C, Sephadex G-200 fractions are stable for at least 6 months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.121 2-deoxy-D-galactose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
1.1.1.121 2-deoxy-D-glucose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
1.1.1.121 D-aldose + NAD+ broad substrate specificity Pseudomonas sp. D-aldonolactone + NADH
-
 ?
1.1.1.121 D-aldose + NAD+ D-aldoses of pyranose ring size Pseudomonas sp. D-aldonolactone + NADH
-
 ?
1.1.1.121 D-allose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
1.1.1.121 D-altrose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
1.1.1.121 D-fucose + NAD+
-
 Pseudomonas sp. D-fucono-delta-lactone + NADH hydrolyzes spontaneously to D-fuconate ?
1.1.1.121 D-galactose + NAD+
-
 Pseudomonas sp. D-galactono-gamma-lactone + NADH
-
 ?
1.1.1.121 D-glucose + NAD+ the most probable produced aldonolactones from this and the following substrates have not been isolated so far Pseudomonas sp. D-glucono-delta-lactone + NADH + H+
-
 r
1.1.1.121 D-glucose + NAD+ beta-anomer preferred over alpha-anomer Pseudomonas sp. D-glucono-delta-lactone + NADH + H+
-
 r
1.1.1.121 D-glucose + NAD+ the most probable produced aldonolactones from this and the following substrates have not been isolated so far Pseudomonas sp. MSU-1 D-glucono-delta-lactone + NADH + H+
-
 r
1.1.1.121 D-mannose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
1.1.1.121 additional information broad substrate specificity Pseudomonas sp. ?
-
 ?
1.1.1.121 additional information substrates are D-aldoses of pyranose ring size Pseudomonas sp. ?
-
 ?
1.1.1.121 additional information L-aldohexoses, ketohexoses, pentoses, trioses, polyols, di- and trisaccharides, derivatives of D-aldohexoses are no substrates Pseudomonas sp. ?
-
 ?
1.1.1.121 additional information NADP+ ineffective as cofactor at concentrations up to 20 mM Pseudomonas sp. ?
-
 ?
1.1.1.121 additional information broad substrate specificity Pseudomonas sp. MSU-1 ?
-
 ?
1.1.1.121 additional information substrates are D-aldoses of pyranose ring size Pseudomonas sp. MSU-1 ?
-
 ?
1.1.1.121 additional information L-aldohexoses, ketohexoses, pentoses, trioses, polyols, di- and trisaccharides, derivatives of D-aldohexoses are no substrates Pseudomonas sp. MSU-1 ?
-
 ?
1.1.1.121 additional information NADP+ ineffective as cofactor at concentrations up to 20 mM Pseudomonas sp. MSU-1 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.121 25
-
 assay at Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.121 55
-
 half-life: 40 s in sodium phosphate buffer, pH 7.0, with D-fucose, D-galactose, D-glucose or D-mannose as substrate Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.121 8 8.5 Tris/HCl buffer Pseudomonas sp.
1.1.1.121 9 10 glycine/NaOH buffer Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.121 7.3 9.3 66% of maximal activity at pH 7.2 and pH 9.3 in Tris/HCl buffer Pseudomonas sp.
1.1.1.121 8.5 10.4 about 80% of maximal activity at pH 8.5 and 10.4 in glycine/NaOH buffer Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.121 NAD+
-
 Pseudomonas sp.
1.1.1.121 NADP+ active at concentrations above 0.02 M Pseudomonas sp.