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Literature summary extracted from

  • Cline, A.L.; Hu, A.S.L.
    The isolation of three sugar dehydrogenases from a pseudomonad (1965), J. Biol. Chem., 240, 4488-4492.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.120 ammonium sulfate precipitation, 40% saturation Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.117 cytoplasm
-
 Pseudomonas sp. 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.117 D-arabinose + NAD(P)+ Pseudomonas sp.
-
 D-arabino-1,4-lactone + NAD(P)H
-
 ?
1.1.1.117 D-arabinose + NAD(P)+ Pseudomonas sp. G6
-
 D-arabino-1,4-lactone + NAD(P)H
-
 ?
1.1.1.120 D-galactose + NADP+ Pseudomonas sp.
-
 D-galactonolactone + NADPH
-
 ?
1.1.1.120 D-galactose + NADP+ Pseudomonas sp. G6
-
 D-galactonolactone + NADPH
-
 ?
1.1.1.121 D-aldose + NAD+ Pseudomonas sp.
-
 D-aldonolactone + NADH
-
 ?
1.1.1.121 D-aldose + NAD+ Pseudomonas sp. G6
-
 D-aldonolactone + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.117 Pseudomonas sp.
-
 G6
-
1.1.1.117 Pseudomonas sp. G6
-
 G6
-
1.1.1.120 Pseudomonas sp.
-
 mutant strain G6
-
1.1.1.121 Pseudomonas sp.
-
 G6
-
1.1.1.121 Pseudomonas sp. G6
-
 G6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.117
-
 Pseudomonas sp.
1.1.1.120 to homogeneity, chromatography techniques Pseudomonas sp.
1.1.1.121 G6 Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.120 cell culture
-
 Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.117 1350
-
-
 Pseudomonas sp.
1.1.1.120 170
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.117 -20°C, acetone-dried cells retain full activity for extended periods of time Pseudomonas sp.
1.1.1.117 4°C, stable as ammonium sulfate precipitate Pseudomonas sp.
1.1.1.117 frozen, purified enzyme stable for many months Pseudomonas sp.
1.1.1.117 lyophilized, purified enzyme stable for many months Pseudomonas sp.
1.1.1.120 4°C, ammonium sulfate precipitate, many months, without appreciable loss of activity Pseudomonas sp.
1.1.1.121 -20°C, acetone-dried cells retain full activity over a long period Pseudomonas sp.
1.1.1.121 5°C, in presence of 2-mercaptoethanol 50% loss of activity after several months Pseudomonas sp.
1.1.1.121 frozen, stable for many months Pseudomonas sp.
1.1.1.121 lyophilized, stable for many months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.117 D-arabinose + NAD(P)+
-
 Pseudomonas sp. D-arabino-1,4-lactone + NAD(P)H
-
 ?
1.1.1.117 D-arabinose + NAD(P)+
-
 Pseudomonas sp. G6 D-arabino-1,4-lactone + NAD(P)H
-
 ?
1.1.1.120 beta-D-galactofuranose + NADP+
-
 Pseudomonas sp. D-galactono-1,4-lactone + NADPH
-
 ?
1.1.1.120 beta-D-galactofuranose + NADP+
-
 Pseudomonas sp. G6 D-galactono-1,4-lactone + NADPH
-
 ?
1.1.1.120 D-galactose + NADP+
-
 Pseudomonas sp. D-galactonolactone + NADPH
-
 ?
1.1.1.120 D-galactose + NADP+
-
 Pseudomonas sp. G6 D-galactonolactone + NADPH
-
 ?
1.1.1.121 D-aldose + NAD+
-
 Pseudomonas sp. D-aldonolactone + NADH
-
 ?
1.1.1.121 D-aldose + NAD+
-
 Pseudomonas sp. G6 D-aldonolactone + NADH
-
 ?
1.1.1.121 D-galactose + NAD+
-
 Pseudomonas sp. D-galactono-gamma-lactone + NADH
-
 ?
1.1.1.121 D-galactose + NAD+
-
 Pseudomonas sp. G6 D-galactono-gamma-lactone + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.117 24
-
 assay at Pseudomonas sp.
1.1.1.121 23
-
 assay at Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.117 50
-
 after 10 min 90% of maximal activity retained Pseudomonas sp.
1.1.1.121 50
-
 rapidly inactivated Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.117 NAD+ NADP+ and NAD+ equally effective Pseudomonas sp.
1.1.1.117 NADP+ NADP+ and NAD+ equally effective Pseudomonas sp.
1.1.1.120 NADP+
-
 Pseudomonas sp.
1.1.1.121 NAD+
-
 Pseudomonas sp.